干酪乳杆菌Zhang分类学地位及潜在益生相关基因分析  

Taxonomic Status and Analysis of Potential Probiotic Genes of Lactobacillus casei Zhang

作　　者：孙佳琦 李伟程 钟智[1,2,3] 张和平[1,2,3] Sun Jiaqi;Li Weicheng;Zhong Zhi;Zhang Heping(Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Inner Mongolia Agricultural University,Hohhot 010018;Key Laboratory of Dairy Products Processing,Ministry of Agriculture and Rural Affairs,Inner Mongolia Agricultural University,Hohhot 010018;Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering,Inner Mongolia Agricultural University,Hohhot 010018)

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室,呼和浩特010018 [2]内蒙古农业大学农业农村部奶制品加工重点实验室,呼和浩特010018 [3]内蒙古农业大学内蒙古乳业生物技术与工程重点实验室,呼和浩特010018

出　　处：《中国食品学报》2023年第8期14-23,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(U22A20540)。

摘　　要：干酪乳杆菌Zhang是本研究团队筛选出的具有优良特性的益生乳酸菌菌株。传统方法难以区分干酪乳杆菌及其近缘物种。因最近乳杆菌的系统分类进行了更新,故导致Zhang的分类地位需重新确认。本研究利用比较基因组学方法分析Zhang的分类学地位、耐药基因、致病性基因和环境抗性基因特征,探究该菌株基因组上携带的细菌素基因簇和潜在益生基因。比较基因组学结果显示Zhang与副干酪乳酪杆菌模式菌株ATCC 25302^(T)的平均核苷酸一致性值是98.49%,通过分类学数据库比对和系统发育分析确认Zhang的分类学地位为副干酪乳酪杆菌。Zhang的基因组不含有耐药基因、致病性基因和环境抗性基因。在其基因组上注释到核心肽、Lan T转运和引导切割及免疫或运输功能等Carnocin细菌素基因簇。此外,Zhang的基因组编码了谷胱甘肽合成、核黄素合成、黏附因子和分泌胞外多糖等潜在益生基因。本研究更新了Zhang的分类学地位为副干酪乳酪杆菌,揭示其不含有耐药基因、致病性基因及环境抗性基因,并注释到多个潜在益生基因,为该菌株进一步开发及产业化提供了数据参考。Lactobacillus casei Zhang is a probiotic lactic acid bacteria strain with excellent properties screened by our research team.It is difficult to distinguish Lactobacillus casei from its relative's species by traditional methods,and the systematic classification of Lactobacillus has recently been updated,resulting in the need to reconfirm the taxonomic status of Zhang.In this study,the taxonomic status,antibiotic resistance genes,virulence genes,and environmental resistance genes of Zhang were characterized using comparative genomics,and the bacteriocin gene cluster and potential probiotic genes carried on the genome of this strain were explored.The comparative genome's results showed that average nucleotide identity value between Zhang and Lacticaseibacillus paracasei ATCC 25302^(T) was 98.49%.The taxonomic status of Zhang was confirmed as Lacticaseibacillus paracasei by comparison with taxonomic database and phylogenetic analysis.Zhang's genome does not contain antibiotic resistance genes,virulence genes,or environmental resistance genes.Carnocin-related bacteriocin gene cluster include core peptide,Lan T transport and guided cleavage,and immune or transport functions were annotated on Zhang's genome.In addition,Zhang's genome encodes potential probiotic genes such as glutathione synthesis,riboflavin synthesis,adhesion factors,and exopolysaccharides secreted.This study updated the taxonomic status of Zhang as Lacticaseibacillus paracasei,revealed that it does not contain antibiotic resistance genes,virulence genes,or environmental resistance genes,and annotated several potential probiotic genes,providing data reference for further development and industrialization of this strain.

关 键 词：副干酪乳酪杆菌 比较基因组学 细菌素基因簇 潜在益生基因 

分 类 号：TS201.3[轻工技术与工程—食品科学]