铁氧化菌菌株EEELCW01的基因组分析及砷转化功能  

Genomic analysis and arsenic transformation of iron-oxidizing strain EEELCW01

作　　者：熊潇然 邹奇 吴川 夏礼兵 潘炜松[1] XIONG Xiaoran;ZOU Qi;WU Chuan;XIALibin;PAN Weisong(College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha,Hunan 410128,China;College of Life Science,South China Normal University,Guangzhou,Guangdong 510006,China;School of Metallurgy and Environment,Central South University,Changsha,Hunan 410083,China)

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]华南师范大学生命科学学院,广东广州510006 [3]中南大学冶金与环境学院,湖南长沙410083

出　　处：《湖南农业大学学报（自然科学版）》2023年第4期428-435,共8页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(42177392)。

摘　　要：以从砷污染农田土壤中分离的1株铁氧化细菌EEELCW01为研究对象,对该菌进行全基因组分析,通过GO、KEGG、COG等数据库比对,预测该菌砷相关基因的功能;采用水培试验,验证该菌的砷转化能力。结果表明:菌株EEELCW01基因组大小为4714242 bp,具有2条大小分别为2065078 bp和2649164 bp的染色体,GC含量为55.99%,染色体上有4588个CDS,包含58个t RNA和12个r RNA;COG数据库注释表明该菌基因功能主要集中在氨基酸转运代谢、无机离子转运代谢等过程,GO数据库注释表明该菌主要包含膜组成部分、氧化还原过程和相关酶活性等功能,KEGG注释显示代谢相关的基因占比最高;菌株EEELCW01基因组中含有多种与砷代谢相关的基因(arsC、ars H、arsB、ars R、acr3、arr A、arxA和arsM);菌株EEELCW01将As(Ⅴ)还原为As(Ⅲ)的能力强,有氧条件下,3 d时还原率达40.1%。可见,可利用该菌株促进砷的生物还原,并联合砷超富集植物进行环境砷污染修复。In this study,the iron-oxidizing bacterium EEELCW01,isolated from As-contaminated soils,underwent comprehensive genome analysis.The potential function of As-related genes was assessed through comparative analysis with GO,KEGG and COG databases,while the As transformation capacity of this strain was investigated via hydroponic experiments.Results unveiled that EEELCW01 possessed a genome size of 4 714 242 bp,encompassing two chromosomes sized at 2 065 078 bp and 2 649 164 bp,respectively,with a GC content of 55.99%.The chromosomes harbored 4588 CDSs,58 t RNAs and 12 r RNAs.COG annotation emphasized gene functions centered on amino acid transport and metabolism,as well as inorganic ion transport and metabolism.GO annotation highlighted functions such as integral membrane components,oxidation-reduction processes and related enzyme activities.KEGG annotation predominantly indicated metabolism-related genes.The strain's genome featured multiple As metabolism-related genes,including ars C,ars H,ars B,ars R,acr3,arr A,arxA and ars M.Hydroponic experiments exhibited the strain's robust capability to reduce As(Ⅴ) to As(Ⅲ),manifesting a reduction rate of 40.1% after 3 days of incubation.In conclusion,through promoting As bioreduction and being in combination with hyperaccumulator,the EEELCW01 could be expected to conduct for the remediation of As-contaminated environments.

关 键 词：砷 铁氧化菌 砷转化 基因组分析 功能注释 

分 类 号：X172[环境科学与工程—环境科学]