气相色谱-质谱法结合气相色谱法鉴别山茶籽油真实属性  

Identification of real properties of camellia seed oil based on gas chromatography-mass spectrometry and gas chromatography

作　　者：滕爽[1,2,3] 鄢永红 沈娟[1,2,3] 孙延勤 沈习习 王玮 TENG Shuang;YAN Yonghong;SHEN Juan;SUN Yanqin;SHEN Xixi;WANG Wei(College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;National Center of Meat Quality and Safety Control,Nanjing Agricultural University,Nanjing 210095,China;Supervision,Inspection and Testing Center for Quality of Meat-products(Nanjing),Ministry of Agriculture and Rural Affairs,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学食品科学技术学院,江苏南京210095 [2]国家肉品质量安全控制工程技术研究中心(南京农业大学),江苏南京210095 [3]农业农村部肉及肉制品质量监督检验测试中心(南京)(南京农业大学),江苏南京210095

出　　处：《食品与发酵工业》2023年第17期267-273,共7页Food and Fermentation Industries

基　　金：国家重点研发计划项目(2018YFC1602804)。

摘　　要：该研究旨在建立一种GC-MS结合GC对掺假山茶籽油进行定性鉴别的方法。该实验基于GC-MS法检测植物油中β-香树脂醇的含量,以实现样品的批量初筛,再结合GC法检测其中油酸与亚油酸的含量进行确证,实现山茶籽油掺假的定性鉴别。结果表明,β-香树脂醇的测定出峰较快、检测周期短、线性关系良好。该方法在低、中、高水平的加标回收率为82.6%~111.5%,相对标准偏差均低于5.36%,可实现植物油样品的批量初筛。结合GC检测其脂肪酸含量进行确证,油酸和亚油酸含量可作为山茶籽油掺假的鉴别指标。该方法可实现山茶籽油中掺入一种或其他几种植物油(花生油、玉米油、菜籽油与大豆油)的定性鉴别,其掺假判定限为10%。This study aimed to establish a method for qualitative identification of adulterated camellia seed oil by gas chromatography-mass spectrometry(GC-MS)combined with gas chromatography(GC).A GC-MS method was established for the rapid detection of the content of β-amyrin in vegetable oil,to realize the batch initial screening of vegetable oil samples.On this basis,the results were confirmed by combining with GC determination of fatty acid content.Results showed that the determination ofβ-amyrin was rapid,the detection period was short,and the linear relationship was good.The average recoveries at low,medium,and high levels were 82.6%-111.5%,and the relative standard deviations(RSD)were all lower than 5.36%.The method could be used for batch screening of vegetable oil samples.In addition,combined with GC determination of fatty acid content to confirm the results,oleic acid and linoleic acid could be used as the identification index of camellia seed oil adulteration.The method is suitable for the qualitative identification of camellia seed oil mixed with one or several other vegetable oils(peanut oil,corn oil,rapeseed oil,and soybean oil),and the adulteration detection limit is 10%.

关 键 词：山茶籽油 掺假 测定与鉴别 β-香树脂醇 脂肪酸 

分 类 号：TS225.16[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.63[轻工技术与工程—食品科学与工程]