正交法优化废弃卤虫冻干保护剂  

Optimization of Freeze-Drying Cryoprotectants for End-of-Life Artemia by Orthogonal Experiment

作　　者：李陇梅[1] 闫东科 吕平[1] 李雪 王永苓 郭媛 LI Longmei;YAN Dongke;LYU Ping;LI Xue;WANG Yonging;GUO Yuan(Tianjin Vocational Institute,Tianjin 300410,China;Jieke(Tianjin)Biomedical Co.,Ltd.,Tianjin 300467,China;Tianjin Haiyou Jiayin Biotechnology Co.,Ltd.,Tianjin 300350,China)

机构地区：[1]天津职业大学,天津300410 [2]杰科(天津)生物医药有限公司,天津300467 [3]天津海友佳音生物科技股份有限公司,天津300350

出　　处：《生物化工》2023年第4期42-45,共4页Biological Chemical Engineering

基　　金：天津市科技计划项目(22YDTPJC00280)。

摘　　要：为提高真空冷冻干燥废弃卤虫的品质,通过单因素实验和正交实验设计,研究了不同质量分数海藻糖、甘露醇、蔗糖和精氨酸对废弃卤虫冻干保持原有营养成分的影响,确定适合废弃卤虫的最佳冻干保护剂配方。结果表明,4种物质的保护效果依次为海藻糖>甘露醇>蔗糖>精氨酸;最佳保护剂配方为20%海藻糖+15%蔗糖+8%甘露醇+2%精氨酸。加入冻干保护剂后,二十二碳六烯酸(DHA)含量为4.8 g/kg,二十碳五烯酸(EPA)含量为2.2 g/kg,维生素C含量为38.3 mg/kg,保存率均超过90%。In order to improve the quality of vacuum freeze-drying end-of-life artemia,single factor experiment and orthogonal experiment design are adopted,the effects of trehalose,mannitol,sucrose and arginine contents on the preservation of physical properties and nutritional components of end-of-life artemia are studied.The optimum formulation of freeze-drying protectant for end-of-life artemia is determined.The results are as follows:the order of the influencing factors is trehalose mannitol sucrose arginine;the best cryoprotectants ingredient is 20%trehalose+15% sucrose+8% mannitol+2% arginine;after adding lyophilized protective agent,the content of DHA,EPA and Vitamin C is 4.8 g/kg,2.2 g/kg and 38.3 mg/kg,respectively,and the preservation rate are more than 90%.

关 键 词：废弃卤虫 真空冷冻干燥 冻干保护剂 正交实验 

分 类 号：S816.9[农业科学—饲料科学]