The long non-coding RNA DANA2 positively regulates drought tolerance by recruitingERF84 to promote JMJ29-mediated histone demethylation  

作　　者：Pengxiang Zhang Reqing He Jun Yang Jingjing Cai Zhipeng Qu Rongxin Yang Jinbao Gu Zhen-Yu Wang David LAdelson Youlin Zhu Xiaofeng Cao Dong Wang 

机构地区：[1]Key Laboratory of Molecular Biology and Gene Engineering in Jiangxi Province,College of Life Science,Nanchang University,Jiangxi 330031,China [2]State Key Laboratory of Plant Genomics and National Center for Plant Gene Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China [3]University of Chinese Academy of Sciences,Beijing 100039,China [4]Department of Molecular and Biomedical Science,School of Biological Sciences,The University of Adelaide,Adelaide,SA 5005,Australia [5]Institute of Nanfan&Seed Industry,Guangdong Academy of Sciences,Guangdong 510316,China

出　　处：《Molecular Plant》2023年第8期1339-1353,共15页分子植物（英文版）

基　　金：supported by the National Natural Science Foundation of China(grants 32070627 and 31960138 to D.W.,31800224 and 32160070 to R.H.,and 31788103 to X.C.);the Chinese Academy of Sciences(Strategic Priority Research Program XDB27030201 to X.C.);the Natural Science Foundation of Jiangxi Province(grant 20171ACB20001 to D.W.).

摘　　要：Tens of thousands of long non-coding RNAs have been uncovered in plants,but few of them have been comprehensively studied for their biological function and molecular mechanism of their mode of action.Here,we show that the Arabidopsis long non-coding RNA DANA2 interacts with an AP2/ERF transcription factor ERF84 in the cell nucleus and then affects the transcription of JMJ29 that encodes a Jumonji C domain-containing histone H3K9 demethylase.Both RNA sequencing(RNA-seq)and genetic analyses demonstrate that DANA2 positively regulates drought stress responses through JMJ29.JMJ29 positively regulates the expression of ERF15 and GOLS2 by modulation of H3K9me2 demethylation.Accordingly,mutation of JMJ29 causes decreased ERF15 and GOLS2 expression,resulting in impaired drought tolerance,in agreement with drought-sensitive phenotypes of dana2 and erf84 mutants.Taken together,these results demonstrate that DANA2 is a positive regulator of drought response and works jointly with the transcriptional activator ERF84 to modulate JMJ29 expression in plant response to drought.

关 键 词：long NON-CODING RNA H3K9 DEMETHYLASE TRANSCRIPTION factor DROUGHT response 

分 类 号：Q94[生物学—植物学]