ICP-MS法测定砷脂摄入对小鼠砷蓄积及矿物元素水平的影响  

Effects of arsenolipids intake on arsenic accumulation and mineral elements in mice determined by ICP-MS

作　　者：陈佳佳 钟映雄 汪卓 李瑞[1] 贾学静 陈建平[1] 刘晓菲 宋兵兵 钟赛意[1,2,3] CHEN Jiajia;ZHONG Yingxiong;WANG Zhuo;LI Rui;JIA Xuejing;CHEN Jianping;LIU Xiaofei;SONG Bingbing;ZHONG Saiyi(College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China;Shenzhen Research Institute,Guangdong Ocean University,Shenzhen 518108,China;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian 116034,China)

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]广东海洋大学深圳研究院,广东深圳518108 [3]大连工业大学海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034

出　　处：《中国渔业质量与标准》2023年第4期45-52,共8页Chinese Fishery Quality and Standards

基　　金：国家自然科学基金面上项目(31972163);“十三五”国家重点研发计划项目(2020YFD0901101);广东省重点领域研发计划项目(2020B1111030004);广东省高校科技创新团队项目(2021KCXTD021)。

摘　　要：砷脂在海洋生物中含量丰富,饮食途径是人们暴露砷脂的主要来源。为初步判断砷脂摄入对小鼠的影响,本研究利用电感耦合等离子体质谱(ICP-MS)法测定3种砷脂(AsHC 332、AsHC 346和AsHC 374)摄入对小鼠砷蓄积以及对小鼠肝肾组织中钾(K)、钠(Na)、钙(Ca)、镁(Mg)、铜(Cu)、锌(Zn)、铁(Fe)、钴(Co)、镍(Ni)、锰(Mn)、硒(Se)和铬(Cr)12种矿物元素水平变化情况。结果表明,3种砷脂摄入后均会在小鼠的心、肝、脾、肺和肾中出现砷蓄积,其中肾脏的砷蓄积作用最明显,肝脏次之。且3种砷脂摄入会显著改变肝脏中12种矿物元素水平变化和肾脏中7种矿物元素水平变化,尤其是Cu、Zn、Se等与抗氧化酶合成相关的元素的紊乱。本研究可初步判断3种砷脂的摄入会对小鼠的肝肾组织造成一定影响,可以为后续砷脂的毒理学相关内容提供实验基础,为海产品风险评估提供一定的数据支撑。Arsenolipids are abundant in marine organisms,and the dietary route may be the main source of arsenolipids exposure in people.To initially determine the effects of arsenolipids intake on mice,inductively coupled plasma-mass spectrometry(ICP-MS)was used to determine the effects of three arsenolipids(AsHC 332,AsHC 346 and AsHC 374)on arsenic accumulation in mice and on changes in the levels of 12 mineral elements,K,Na,Ca,Mg,Cu,Zn,Fe,Co,Ni,Mn,Se and Cr,in the liver and kidney tissues of mice.The results showed that arsenic accumulation occurred in the heart,liver,spleen,lung,and kidney of mice after the intake of the three arsenolipids,with the kidney having the most significant effect on arsenic accumulation,followed by the liver.In addition,the input of the three arsenolipids significantly changed the level of 12 mineral elements in the liver and 7 mineral elements in the kidney,especially the disorders of Cu,Zn,Se,and other elements related to antioxidant enzymes.This study can initially determine that the intake of three arsenolipids will cause specific effects on mice's liver and kidney tissues,which can provide an experimental basis for the subsequent toxicology-related contents of arsenolipids and provide specific experimental data for the risk assessment of seafood.

关 键 词：电感耦合等离子体质谱法 砷脂 矿物元素 肾脏 肝脏 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]