Cyclophilin D as a potential therapeutic target of liver ischemia/reperfusion injury by mediating crosstalk between apoptosis and autophagy  

作　　者：Mengjiao Yang Zhihui Wang Jin Xie MdReyad‐ul‐Ferdous Siying Li Yongfeng Song 

机构地区：[1]Department of Endocrinology,Shandong Provincial Hospital Affiliated to Shandong First Medical University,Jinan,Shandong,China [2]Shandong Key Laboratory of Endocrinology and Lipid Metabolism,Jinan,Shandong,China [3]Shandong Institute of Endocrine and Metabolic Diseases,Jinan,Shandong,China [4]Department of Endocrinology,Shandong Provincial Hospital,Cheeloo College of Medicine,Shandong University,Jinan,Shandong,China [5]Department of Endocrinology and Metabolism,Central Hospital Affiliated to Shandong First Medical University,Jinan,Shandong,China

出　　处：《Chronic Diseases and Translational Medicine》2023年第3期238-249,共12页慢性疾病与转化医学（英文版）

基　　金：Independently cultivates innovation team program of Jinan,China,Grant/Award Number:2021GXRC048;Shandong Provincial Natural Science Foundation,Grant/Award Number:ZR2020ZD14;National Natural Science Foundation of China,Grant/Award Number:82270922;National Key Research and Development Program of China,Grant/Award Number:2022YFA0806100。

摘　　要：Background:Liver ischemia/reperfusion(I/R)injury is a complex and multifactorial pathophysiological process.It is well recognized that the membrane permeability transition pore(mPTP)opening of mitochondria plays a crucial role in cell death after I/R injury.Cyclophilin D(CypD)is a critical positive regulator of mPTP.However,the effect of CypD on the pathogenesis of liver I/R injury and whether CypD is a potential therapeutic target are still unclear.Methods:We constructed liver-specific CypD knockout and AAV8-peptidyl prolyl isomerase F(PPIF)overexpression mice.Then,a 70%liver I/R injury model was established in mice,with 90 min of ischemia and 6 h of reperfusion.The liver function was detected by the level of serum glutamic pyruvic transaminase(alanine transaminase)and glutamic oxaloacetic transaminase(aspartate aminotransferase),the liver damage score and degree of necrosis were measured by hematoxylin and eosin(H&E)staining of liver tissues.Reactive oxygen species(ROS)staining,apoptosis,and autophagy-related molecules were used to detect apoptosis and autophagy during liver I/R.Results:The liver-specific knockout of CypD alleviated necrosis and dysfunction in liver I/R injury,by reducing the excessive production of ROS,and inhibiting cell apoptosis and autophagy.On the contrary,overexpression of CypD exacerbated I/R-induced liver damage.Conclusion:We found that the downregulation of CypD expression alleviated liver I/R injury by reducing apoptosis and autophagy through caspase-3/Beclin1 crosstalk;in contrast,the upregulation of CypD expression aggravated liver I/R injury.Therefore,interfering with the expression of CypD seems to be a promising treatment for liver I/R injury.

关 键 词：APOPTOSIS AUTOPHAGY cyclophilin D ISCHEMIA/REPERFUSION NECROSIS reactive oxygen species 

分 类 号：R575[医药卫生—消化系统]