冠突散囊菌不同生殖方式的比较转录组学分析  

Comparative Transcriptomic Analysis of Different Reproductive Types of Euro⁃tium cristatum

作　　者：胡治远 刘石泉 徐正刚 董萌 HU Zhiyuan;LIU Shiquan;XU Zhenggang;DONG Meng(Hunan Provincial Key Lab of Dark Tea and Jin-hua,Hunan City University,Yiyang,413000;College of Forestry,Northwest A&F University,Yangling,712100)

机构地区：[1]湖南城市学院黑茶金花湖南省重点实验室,益阳413000 [2]西北农林科技大学林学院,杨凌712100

出　　处：《基因组学与应用生物学》2023年第4期400-417,共18页Genomics and Applied Biology

基　　金：湖南省自然科学基金项目(2021JJ40021);湖南省教育厅优秀青年项目(20B112)共同资助

摘　　要：冠突散囊菌(Eurotium cristatum)是黑茶中的优势微生物,其存在差异较大的有性型和无性型生殖方式,本研究旨在分析冠突散囊菌两种生殖方式下的基因表达差异,为其生殖发育研究提供数据。对冠突散囊菌有性型和无性型的菌体进行转录组测序,分析与两种生殖方式相关的基因及其表达机制。结果表明,两种类型菌体转录组序列中共发现了3810个差异表达基因(DEGs),主要富集在次生代谢产物、生物膜、氨基酸的生物合成等相关代谢通路中,负责调控氧化还原反应、催化活性、金属离子结合以及跨膜转运等。通过对DEGs进行功能分析,从中筛选了chsD、chsC、HOP1、DMC1、MAT1-2和wetA等66个可能在冠突散囊菌生殖过程中起关键调控作用的基因,主要在孢子壁合成、性别分化、钙离子代谢途径等方面行使功能,其中MAT1-2、wetA已被验证在冠突散囊菌性别分化及孢子形成过程中具有重要作用。本研究为冠突散囊菌生殖调控及高效产孢发酵技术提供了基础。Eurotium cristatum is the dominant microorganism in dark tea,which has different sexual and asexual reproduction types.The aim of this study is to analyze the differences in gene expression between two reproductive types of Eurotium cristatum,and to provide data for the study of its reproductive development.Transcriptome sequencing was performed on the sexual and asexual strains ofEurotium cristatum to analyze the genes related to the two reproduction types and their expression mechanisms.The results showed that a total of 3810 differentially expressed genes were found in the transcriptome sequences of the two types of hyphae,which were mainly enriched in secondary metabolites,bio-membrane,amino acid biosynthesis,and other related metabolic pathways,and were responsible for the regulation of redox reaction,catalytic activity,metal ion binding,and transmembrane transport,among others.Performed functional analyses on differentially expressed genes to screen 66 genes that might be influential in regulatingEurotium cristatum reproduction,e.g.,chsD,chsC,HOP1,DMC1,MAT1-2 andwetA,which mainly manifest their functions during spore wall synthesis,gender differentiation and calcium ion metabolic pathway.Among them,MAT1-2 andwetA have been proved to play an important role inEurotium cristatum's gender differentiation and sporulation.This study can serve as a fundamental reference for the regulation ofEurotium cristatum reproduction and highly-yield fermentation procedure for sporulation.

关 键 词：冠突散囊菌 生殖方式 转录组测序 基因挖掘 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]