上尿路尿路上皮癌患者尿液菌群特征分析  

Characteristics of the urinary microbiota in UTUC patients

作　　者：聂亮 程立 杨成林 张昊 张振兴 黄后宝 郝宗耀[2] 梁朝朝[2] 卓栋 Nie Liang;Cheng Li;Yang Chenglin;Zhang Hao;Zhang Zhenxing;Huang Houbao;Hao Zongyao;Liang Chaozhao;Zhuo Dong(Department of Urology,Yjishan Hospital,the First Affiliated Hospital of Wannan Medical College,Wuhu 241000,China;Department of Urology,the First Affiliated Hospital of Anhui Medical University,Institute of Urology&Anhui Province Key Laboratory of Genitourinary Diseases,Hefei 230022,China)

机构地区：[1]皖南医学院第一附属医院弋矶山医院泌尿外科,芜湖241000 [2]安徽医科大学第一附属医院泌尿外科、泌尿生殖系统疾病安徽省重点实验室,合肥230022

出　　处：《中华泌尿外科杂志》2023年第9期665-669,共5页Chinese Journal of Urology

基　　金：安徽省自然科学基金(1908085MH285)。

摘　　要：目的探讨上尿路尿路上皮癌(UTUC)患者的尿路菌群特征。方法收集皖南医学院.第一附属医院弋矶山医院2021年7月至2022年7月收治的23例UTUC患者(UTUC组)和22例良性疾病患者(对照组)的尿液样本。UTUC组与对照组年龄[(60.9±5.7)岁与(61.4±8.8)岁]、性别(男/女:15/8与9/13)、体质量指数[(22.9±1.8)kg/m^(2)与(23.4±1.7)kg/m^(2)]差异均无统计学意义(P>0.05)。使用NovaSeq6000测序平台对尿液样本微生物16SrRNA的V4区测序,使用QLLME2软件处理所得数据。采用Shannon指数、辛普森指数和Chaol指数分析组间α多样性;使用非加权主坐标分析(PCoA)进行组间β多样性分析。线性判别分析(LEISe)鉴定组间丰度存在差异的菌群,以.线性判别分析值(LDA)>2为有显著差异。结果UTUC组较对照组的Chaol指数(703.12±265.54与506.20±214.02)和Shannon指数(5.61±1.85与5.07±1.34)均明显增高(P<0.05),UTUC组较对照组患者尿液微生物的α多样性升高,但β多样性差异无统计学意义(P=0.161)。UTUC组中拟杆菌科、瘤胃球菌科、氨基酸球菌科、栖热菌科、韦荣球菌科和红蝽菌科丰度较高,且与对照组存在显著差异(LDA>2)。对UTUC患者进一步行亚组分析,肌层浸润组与非肌层浸润组的Chaol指数(706.44±271.84与784.09±272.72)、Shannon指数(6.04±1.30与5.91±1.67)和辛普森指数(0.94±0.08与0.89±0.22)差异均无统计学意义(P>0.05),即两组菌群a多样性差异无统计学意义,但β多样性差异有统计学意义(P=0.047)。非肌层浸润患者的尿液微生物群中γ-变形菌纲、痤疮丙酸杆菌属、红球菌属、放线菌科丰度更高,且与肌层浸润患者存在显著差异(LDA>2)。结论本研究结果显示UTUC患者较非UTUC患者尿液微生物群更丰富,且拟杆菌科、瘤胃球菌科、氨基酸球菌科、栖热菌科、韦荣球菌科和红蝽菌科在UTUC患者尿液中丰度更高。非肌层浸润患者较肌层浸润患者尿液中γ-变形菌纲、痤疮丙酸杆菌Objective To analyzed the urinary microbiota characteristics of upper tract urothelial carcinoma(UTUC)patients.Methods Urine samples were collected from 23 patients with UTUC(UTUC group)and 22 patients with benign diseases(control group)admitted to Yijishan Hospital,the First Afriliated Hospital of Wannan Medical College from July 2021 to July 2022.The differences in age[(60.9±5.7)years vs.(61.4±8.8)years],sex(male/female:15/8 vs.9/13),and body mass index[(22.9±1.8)kg/m^(2)vs.(23.4±1.7)kg/m^(2)] between the UTUC group and the control group were not statistically significant(P>0.05).The V4 region of the 16S rRNA of urinary microorganisms was sequenced using the Illumina NovaSeq6000,and the results were processed using QLLME2.Differences inα-diversity between groups were analyzed by using the Shannon,Simpson,and Chaol indices.Differences inβ-diversity between groups were analyzed by using unweighted principal coordinates analysis(PCoA)Linear discriminant analysis Effect Size(LEfSe)was used to identify the bacterial taxa with different abundances between groups.Significant differences were defined as LDA>2.Results The Chaol index(703.12±265.54 vs.506.20±214.02)and Shannon index(5.61±1.85 vs.5.07±1.34)were significantly higher in the UTUC group compared to that in the control group(P<0.05).Theα-diversity of urinary microbes was elevated in the UTUC group compared to that in the control group but the difference inβ-diversity was not statistically significant(P=0.161).The enrichment of Bacteroidaceae,Ruminococcaceae,Acidaminococcaceae,Thermaceae,Erysipelatoclostridiaceae,and Coriobacteriaceae abundance was higher in the urine of UTUC patients(LDA>2).Further subgrouping analyses of the UTUC patients showed that the differences in Chao1 index(706.44±271.84 vs.784.09±272.72),Shannon index(6.04±1.30 vs.5.91±1.67),and Simpson index(0.94±0.08 vs.0.89±0.22)between the muscle-invasive group and the non-muscle-invasive group were not statistically significant(P>0.05).The difference in α-diversity between musc

关 键 词：上尿路尿路上皮癌 尿液微生物 基因测序 线性判别分析 

分 类 号：R737.1[医药卫生—肿瘤]