凝结芽孢杆菌CGMCC 9951对小鼠溃疡性结肠炎的保护作用  

The protective effect of Bacillus coagulans CGMCC 9951on ulcerative colitis in mice

作　　者：高涵 吴影[1,2,3] 铁珊珊 赵丽娜 李欣[1,2,3] 曹力 古绍彬[1,2,3] GAO Han;WU Ying;TIE Shanshan;ZHAO Lina;LI Xin;CAO Li;GU Shaobin(College of Food and Bioengineering,Henan University of Science and Technology,Luoyang 471000,China;Henan Engineering Research Center of Food Microbiology,Luoyang 471023,China;National Demonstration Center for Experimental Food Processing and Safety Education,Luoyang 471023,China)

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471000 [2]河南省食品微生物工程技术研究中心,河南洛阳471023 [3]食品加工与安全国家级实验教学示范中心,河南洛阳471023

出　　处：《食品与发酵工业》2023年第19期90-96,共7页Food and Fermentation Industries

基　　金：河南省自然科学基金面上项目(222300420429);河南省科技攻关计划(212102110326)。

摘　　要：该研究旨在探讨凝结芽孢杆菌CGMCC 9951对5%葡聚糖硫酸钠(dextran sulfate sodium,DSS)诱导的小鼠溃疡性结肠炎的干预效应。30只昆明小鼠随机分为对照组、模型组及CGMCC 9951低、中、高干预组,在适应性饲养7 d后,实验第1、2周对照组与模型组灌胃无菌生理盐水,低、中、高干预组分别灌胃3×10^(6)、3×10^(7)及3×10^(8) CFU/mL CGMCC 9951菌悬液,实验第3周除对照组外每组给予5%DSS溶液进行造模,7 d后禁食12 h处死小鼠。与模型组相比,高剂量CGMCC 9951使小鼠体重增加8.59%,结肠长度增加38.55%;疾病活动指数降低53.33%,结肠炎性细胞浸润等黏膜损伤减轻46.43%;同时谷胱甘肽浓度升高181.89%、总超氧化物歧化酶活力升高48.73%、丙二醛含量下降55.41%,表明氧化应激程度得到改善;且促炎因子水平降低24.87%、抗炎因子水平提高46.81%,表明炎症反应的减轻;进一步研究发现,小鼠血清脂多糖水平降低16.57%,二胺氧化酶活力上升74.49%,有效维护了肠道机械屏障。该研究结果说明高剂量的CGMCC 9951对DSS诱导的小鼠结肠炎有良好的保护效果。The purpose of this study was to investigate the intervention effect of Bacillus coagulans CGMCC 9951 on 5%dextran sodium sulfate(DSS)-induced ulcerative colitis in mice.Thirty KM mice were randomly divided into control group,model group and low,medium and high intervention groups of CGMCC 9951.After 7 d of adaptive feeding,the control group and model group were given 0.3 mL of sterile normal saline,and low,medium,and high intervention groups were given 3×10^(6),3×10^(7),and 3×10^(8) CFU/mL of CGMCC 9951 bacterial suspension.In the third week of the experiment,each group except the control group was given 5%DSS solution for modeling.DSS was stopped at 7 d after modeling,and mice were sacrificed after fasting for 12 h.The results showed that compared with the model group,high dose CGMCC 9951 increased body weight by 8.59%and colon length by 38.55%in mice,decreased DAI by 53.33%and reduced mucosal damage such as colonic inflammatory cell infiltration by 46.43%.Moreover,glutathione concentration increased 181.89%,total superoxide dismutase activity increased 48.73%and malondialdehyde content decreased 55.41%indicating improvement in the degree of oxidative stress.Then,after intervention with GCMCC9951,a 24.87%decrease of pro-inflammatory cytokines and a 46.81%increase of anti-inflammatory cytokines indicated a reduction in the inflammatory response.Further studies showed that the concentration of lipopolysaccharide decreased by 16.57%and the activity of diamine oxidase increased by 74.49%in mice,which effectively maintained the colon barrier.The results showed that high dose of CGMCC 9951 had a good intervention effect on DSS-induced colitis in mice,and provided a scientific basis for the protection of B.coagulans CGMCC 9951 on colitis.

关 键 词：凝结芽孢杆菌 溃疡性结肠炎 葡聚糖硫酸钠 炎症因子 肠道屏障 

分 类 号：R574.62[医药卫生—消化系统]