Sulforaphane prevents LPS‑induced inflammation by regulating the Nrf2‑mediated autophagy pathway in goat mammary epithelial cells and a mouse model of mastitis  

作　　者：Dan Shao Wenxiang Shen Yuyang Miao Zhen Gao Menghao Pan Qiang Wei Zuoting Yan Xiaoe Zhao Baohua Ma 

机构地区：[1]Key Laboratory of Animal Biotechnology of the Ministry of Agriculture,College of Veterinary Medicine,Northwest A&F University,Yangling 712100,Shaanxi,China [2]Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Science,Lanzhou 730050,China

出　　处：《Journal of Animal Science and Biotechnology》2023年第5期2093-2106,共14页畜牧与生物技术杂志（英文版）

基　　金：supported by Fuping County Dairy Goat High-efficiency Breeding Technology R&D and Extension Application Project(No.K3380216101);the Dairy Goat High-efficiency Breeding Technology Research and Application Project(No.K4040121023).

摘　　要：Background Mastitis not only deteriorates the composition or quality of milk,but also damages the health and pro-ductivity of dairy goats.Sulforaphane(SFN)is a phytochemical isothiocyanate compound with various pharmacologi-cal effects such as anti-oxidant and anti-inflammatory.However,the effect of SFN on mastitis has yet to be elucidated.This study aimed to explore the anti-oxidant and anti-inflammatory effects and potential molecular mechanisms of SFN in lipopolysaccharide(LPS)-induced primary goat mammary epithelial cells(GMECs)and a mouse model of mastitis.Results In vitro,SFN downregulated the mRNA expression of inflammatory factors(tumor necrosis factor-α(TNF-α),interleukin(IL)-1βand IL-6),inhibited the protein expression of inflammatory mediators(cyclooxygenase-2(COX2),and inducible nitric oxide synthase(iNOS))while suppressing nuclear factor kappa-B(NF-κB)activation in LPS-induced GMECs.Additionally,SFN exhibited an antioxidant effect by increasing Nrf2 expression and nuclear translocation,up-regulating antioxidant enzymes expression,and decreasing LPS-induced reactive oxygen species(ROS)produc-tion in GMECs.Furthermore,SFN pretreatment promoted the autophagy pathway,which was dependent on the increased Nrf2 level,and contributed significantly to the improved LPS-induced oxidative stress and inflammatory response.In vivo,SFN effectively alleviated histopathological lesions,suppressed the expression of inflammatory factors,enhanced immunohistochemistry staining of Nrf2,and amplified of LC3 puncta LPS-induced mastitis in mice.Mechanically,the in vitro and in vivo study showed that the anti-inflammatory and anti-oxidative stress effects of SFN were mediated by the Nrf2-mediated autophagy pathway in GMECs and a mouse model of mastitis.Conclusions These results indicate that the natural compound SFN has a preventive effect on LPS-induced inflam-mation through by regulating the Nrf2-mediated autophagy pathway in primary goat mammary epithelial cells and a mouse model of mastitis,which may improve preventio

关 键 词：AUTOPHAGY Goat mammary epithelial cells INFLAMMATION NRF2 Oxidative stress SULFORAPHANE 

分 类 号：S859[农业科学—临床兽医学]