Identification of novel targets and mechanisms of wogonin on lung cancer,bladder cancer,and colon cancer  

作　　者：Lin Zhou Yunran Hu Changxing Gao Congci Yu Zhiting Sun Weihong Ge Hui Yang 

机构地区：[1]Department of Pharmacy,Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing 210008,China [2]China Pharmaceutical University Nanjing Drum Tower Hospital,Nanjing 210008,China [3]Hospital for Skin Disease,Institute of Dermatology,Chinese Academy of Medical Sciences and Peking Union Medical College,Nanjing 210042,China [4]Department of Scientific Research,Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing 210008

出　　处：《Journal of Future Foods》2024年第3期267-279,共13页未来食品学报（英文）

基　　金：supported by the Fundamental Research Funds for the Central Universities(3332022085,3332022083,3332022084);the National Natural Science Foundation of China(82204720)。

摘　　要：Wogonin(WOG)has been demonstrated to have anti-cancer activity,but the mechanisms remain unclear.In this study,new targets of WOG were predicted for lung cancer,bladder cancer,and colon cancer by using bioinformatics methods.wOG might primarily suppress cancers via regulating arachidonic acid,Ras,MAPK,linoleic acid,PI3K Akt,and folate biosynthesis pathways.3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS)assay showed that WOG inhibited the proliferation of A549 cells.Real-time quantitative reverse transcription PCR(RT-qPCR)results indicated that anti-lung cancer effect of WOG was achieved by regulating the expression of 18 target genes,including AKRIBI0,AKRIC3,BDNF,CAVI,CXCL2,CYP2B6,CYP4F3,DAO,EGF,ENO3,IL6,PLA2GlB,PLA2G2F,PLA2G4A,PTGES,SLCOIBI,SLCOIB3,and TFAP2A.The Kaplan-Meier survival curves further confirmed that DAO,PLA2G1B,SLCO1B3 and TFAP2A were essential targets via which WOG affected lung cancer survival.Moreover,BDNF,FGF2,and PTGS1 were predicted to be the targets via which WOG alleviated cancer proliferation and invasion in bladder cancer.As for colon cancer,WOG might induce autophagy and inhibit proliferation by down-regulating NTF4 and TH.The study will provide clue for using wOG as a promising antineoplastic agent in basic and translational research,and bring light to the application of herbs containing WOG as food supplements.

关 键 词：WOGONIN TARGETS Lung cancer Bladder cancer Colon cancer 

分 类 号：R734.2[医药卫生—肿瘤]