丛毛红曲霉工程菌TI-25高产莫纳可林K发酵工艺优化  

Optimization of Fermentation Process for High Monacolin K Production by Monascus pilosus Engineering Strain TI-25

作　　者：张亚如 肖丽酾 陈丽晨 叶燕芳 郑政淮 黄志伟[1,2] ZHANG Yaru;XIAO Lishi;CHEN Lichen;YE Yanfang;ZHENG Zhenghuai;HUANG Zhiwei(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China)

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]福建农林大学福建省特种淀粉品质科学与加工技术重点实验室,福建福州350002

出　　处：《食品研究与开发》2023年第20期115-121,共7页Food Research and Development

基　　金：福建省科技计划对外合作项目(2019I0008);福建农林大学科技创新专项基金项目(CXZX2023)。

摘　　要：为优化丛毛红曲霉工程菌TI-25高产莫纳可林K(monacolin K,MK)的发酵工艺,以菌株TI-25作为研究对象,以MK产量为评价指标,采用固态发酵方式,在单因素试验基础上,采用响应面优化设计,探究丛毛红曲霉工程菌TI-25高产MK的最佳发酵工艺。结果表明:发酵瓶装米量、初始加水量和发酵变温时间均会显著影响发酵产物的MK产量,而发酵时间、种子液培养基初始pH值和种子液接种量对发酵产物的MK产量的影响较小。因此,选取发酵瓶装米量、初始加水量、发酵变温时间3个因素进行响应面优化设计。优化结果表明,菌株TI-25固态发酵产MK的最佳发酵工艺为发酵瓶装米量20 g/350 mL,初始加水量32.38 mL,30℃培养3 d后,变温为28℃继续培养12 d,种子液接种量10%,种子液培养基初始pH4.5,在此条件下发酵培养,发酵产物红曲米中的MK产量高达635.12 mg/kg。This study aimed to optimize the fermentation process for high monacolin K(MK)production by Monascus pilosus engineering strain TI-25.The strain TI-25 was taken as the research object,and the MK yield was taken as the evaluation index.The solid-state fermentation method was used to explore the optimal fermentation process for high MK production by Monascus pilosus engineering strain TI-25 on the basis of a single factor experiment and response surface optimization design.The results showed that the amount of fermented bottled rice,initial water addition,and temperature change time of fermentation would significantly affect the MK yield of fermentation products.The fermentation days,initial pH value of culture medium with seed solution,and inoculation amount of seed solution had little effect on the MK yield of fermentation products;therefore,the response surface optimization design was carried out by selecting three factors:the amount of fermented bottled rice,the initial water addition,and the fermentation days.The optimization results showed that the optimal fermentation conditions for MK production by solid-state fermentation of engineering strain TI-25 were as follows:The amount of fermented bottled rice was 20 g/350 mL;the initial water addition was 32.38 mL;the temperature was changed from 30℃for curing of 3 d to 28℃for curing of 12 d;the inoculation amount of seed solution was 10%,and the initial pH value of culture medium with seed solution was 4.5.In these conditions,the content of MK in Monascus rice could reach 635.12 mg/kg.

关 键 词：丛毛红曲霉 工程菌 固态发酵 莫纳可林K 发酵工艺 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] TQ460.1