春生田头菇粗多糖超声辅助提取及抗氧化性测定  

Ultrasound-assisted extraction and antioxidant study of crude polysaccharide from Agrocybe praecox

作　　者：韩晓磊 吕慧英[2] 梁珏钦 范伟 刘韬 黄志群 杨国舜 熊智[1] HAN Xiaolei;LU Huiying;LIANG Jueqin;FAN Wei;LIU Tao;HUANG Zhiqun;YANG Guoshun;XIONG Zhi(College of Biodiversity Conservation,Southwest Forestry University,Kunming,Yunnan 650224,China;Institute of Agricultural Product Processing,Hunan Academy of Agricultural Sciences,Changsha,Hunan 410125,China;College of Food Science and Technology,Hunan Agricultural University,Changsha,Hunan 410128,China;Agriculture and Rural Bureau of Yuanjiang City,Yiyang,Hunan 413100,China;Hunan Lugu Biotechnology Co.,Ltd.,Yiyang,Hunan 413100,China;Hunan Boda Tianeng Industry Co.,Ltd.,Yiyang,Hunan 413100,China)

机构地区：[1]西南林业大学生物多样性保护学院,云南昆明650224 [2]湖南省农业科学院农产品加工研究所,湖南长沙410125 [3]湖南农业大学食品科学技术学院,湖南长沙410128 [4]沅江市农业农村局,湖南益阳413100 [5]湖南芦菇生物科技有限公司,湖南益阳413100 [6]湖南博大天能实业股份有限公司,湖南益阳413100

出　　处：《食品与机械》2023年第9期169-176,共8页Food and Machinery

基　　金：湖南省农业科技创新资金项目(编号:2022CX93);湖南创新型省份建设专项—科技特派员服务乡村振兴项目(编号:2022NK4280)。

摘　　要：目的:为高效利用洞庭湖特色芦苇食用菌资源,开发食用菌多糖功能性食品。方法:以芦苇食用菌春生田头菇为试验原料,以多糖提取率为指标,采用超声辅助热水提取法进行粗多糖提取,以液料比、超声时间、超声功率、热水浸提温度、热水浸提时间为单因素条件进行试验,依据Box-Behnken中心组合试验设计建立数学模型,优化春生田头菇粗多糖的提取工艺条件,并考察春生田头菇粗多糖对DPPH自由基、ABTS自由基的清除能力。结果:结合实际,最佳提取工艺条件为料液比1∶50(g/mL)、超声提取时间20 min、超声功率150 W、热水浸提温度80℃、热水浸提时间4 h。在此工艺条件下,春生田头菇粗多糖提取率为5.08%。提取的粗多糖对DPPH自由基和ABTS自由基清除率分别为55.05%,58.47%,半抑制浓度IC50为1.03,0.28 mg/mL。结论:春生田头菇多糖在超声辅助热水提取法最佳工艺参数下,提取得率较高,同时该粗多糖具有一定的体外抗氧化能力。Objective:In order to explore the extraction method and antioxidant activity of crude polysaccharide from edible fungus resources of reed.Methods:The characteristic Agrocybe praecox of reed edible fungus of Dongting Lake District was used as raw material,the extraction rate of polysaccharide was used as the index,and the extraction was carried out by ultrasonic-assisted hot water alcohol precipitation method.After taking the liquid-material ratio,ultrasonic extraction time,ultrasonic extraction power,hot water extraction temperature,hot water extraction time as the factors to finish the single factor experiments.Then the experiments was optimized by Box-Behnken center combined experimental design for the crude polysaccharide extraction process of Agrocybe praecox.The scavenging abilities of the crude polysaccharide on DPPH·free radical and ABTS+·free radical were also studied.Results:The results showed that the optimal extraction conditions were determined:the ratio of solid to liquid of 1∶50(g/mL),ultrasonic extraction time of 20 min,eextraction power of 150 W,Hot water extraction temperature of 80℃,extraction time of 4 h.Under the conditions,the extraction rate of crude polysaccharide was 5.08%.The scavenging rates of DPPH·and ABTS+·were 55.05%and 58.47%,respectively.The semi-inhibitory concentrations of IC 50 were 1.03 and 0.28 mg/mL,respectively.Conclusion:Under the optimal process parameters of ultrasonic-assisted hot water extraction,the extraction rate of polysaccharide is high,and the crude polysaccharide has a certain antioxidant activity in vitro.

关 键 词：春生田头菇 粗多糖 超声辅助 热水浸提 抗氧化性 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]