金瓜多糖不同分级组分的抗氧化和降血糖活性  

Antioxidant and hypoglycemic activities of different graded fractions of polysaccharide from goldenmelon

作　　者：陈冰洁 乔勇进[1,2] 王晓 王春芳[1,2] 刘贵阁 CHEN Bing-jie;QIAO Yong-jin;WANG Xiao;WANG Chun-fang;LIU Gui-ge(Institute of Crop Breeding and Cultivation,Shanghai Academy of Agriculture Sciences,Shanghai 201403,China;Agri-Food Storage and Processing Engineering Technology Research Center of Shanghai,Shanghai 201403,China)

机构地区：[1]上海市农业科学院作物育种栽培研究所,上海201403 [2]上海农产品保鲜加工工程技术研究中心,上海201403

出　　处：《食品与机械》2023年第7期165-171,共7页Food and Machinery

基　　金：上海市农业科学院作物所创新基金(编号:作创字[2021]CX-04);上海市农产品保鲜加工专业技术服务平台(编号:21 DZ2292200)。

摘　　要：目的:研究金瓜多糖不同分级组分的理化特性、结构、抗氧化性和降血糖活性。方法:采用水提醇沉法分级纯化得到4种金瓜多糖(CP-40、CP-60、CP-70、CP-80),通过高效液相色谱、傅里叶变换红外光谱等对其分子量、单糖组成、基团构成、抗氧化和降血糖活性进行研究。结果:金瓜多糖组分随着乙醇体积分数的增加,总糖含量升高,糖醛酸含量降低。4种多糖分子量不同,单糖组成相同但组成比例不同,且均具有多糖类物质的特征吸收峰。金瓜多糖体外抗氧化能力和降血糖活性存在明显的量—效关系,CP-70对DPPH自由基、羟自由基和超氧阴离子自由基清除作用高于其他组,抗氧化性最强,CP-80对α-葡萄糖苷酶和α-淀粉酶抑制作用最强,降血糖活性最佳。结论:金瓜多糖具有良好的抗氧化性和降血糖活性。Objective:The study aimed to research the physicochemical properties,structure,antioxidant activity and hypoglycemic activity of different fractions of polysaccharide of goldenmelon.Methods:Four kinds of goldenmelon polysaccharides(CP-40,CP-60,CP-70,CP-80)were obtained by graded purification of water extraction and alcoholic precipitation.Their molecular weight,monosaccharide composition,group composition,antioxidant and hypoglycemic activities were investigated by high performance liquid chromatography and fourier transform infrared spectroscopy.Results:With the increase of ethanol volume fraction,the total sugar content increased and the glucuronic acid content decreased.The molecular weights of these four polysaccharides were different,which indicated by the same composition of monosaccharides but different composition ratios,and they all had the characteristic absorption peaks of polysaccharides.The antioxidant capacity and hypoglycemic activity of the polysaccharides in vitro had obvious quantity-effect relationships,CP-70 had the strongest antioxidant effect on DPPH radicals,hydroxyl radicals and superoxide anions scavenging than other groups,while CP-80 had the best hypoglycemic activity since it had the strongest inhibitory effects onα-glucosidase andα-amylase.Conclusion:The polysaccharide of goldenmelon had good antioxidant properties and hypoglycemic activity.

关 键 词：金瓜 多糖 体外抗氧化性 降血糖活性 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]