黑木耳β-葡聚糖/纳米硒复合物(DNT-Se)的制备工艺优化及活性评价  

Optimization of the Preparation Process and Evaluation of the Activity ofβ-Glucan/Nano-selenium Complex(DNT-Se)from Black Fungus

作　　者：胡程 宋浩颖 常聪 邱振鹏 郑国华 孟燕 HU Cheng;SONG Haoying;CHANG Cong;QIU Zhenpeng;ZHENG Guohua;MENG Yan(College of Pharmacy,Hubei University of Chinese Medicine,Wuhan 432500,China;Key Laboratory of Chinese Medicine Resource and Compound Prescription,Hubei University of Chinese Medicine,Wuhan 432500,China)

机构地区：[1]湖北中医药大学药学院,湖北武汉432500 [2]湖北中医药大学中药资源与中药复方教育部重点实验室,湖北武汉432500

出　　处：《食品工业科技》2023年第21期162-170,共9页Science and Technology of Food Industry

基　　金：国家自然科学基金青年项目(31900919);国家自然科学基金区域创新发展联合基金重点项目(U21A20297);湖北中医药大学校级杰出青年基金(2022ZZXJ006)。

摘　　要：探索黑木耳β-葡聚糖/纳米硒复合物(DNT-Se)的最佳制备条件,并探究其安全性。以多糖(DNTs)浓度、维生素C(V_(C))与亚硒酸钠配比、亚硒酸钠浓度、反应时间、反应温度作为实验因素,采用双波长比色法及马尔文粒径测定法表征DNT-Se中Se NPs的粒径变化,以Se NPs的粒径大小为评价指标,采用单因素及正交试验设计的方法考察DNT-Se的制备工艺,并采用CCK-8法评价DNT-Se对293T人胚胎肾细胞和AML-12小鼠正常肝细胞的毒性。DNTs浓度1.0 mg/mL,维生素C与亚硒酸钠配比为6:1,亚硒酸钠浓度为1.0 mg/mL、反应时间12 h、反应温度25℃为制备DNT-Se的最佳条件。该条件下制备的DNT-Se中Se NPs的粒径大小均值为34.50 nm,细胞增殖实验结果表明,DNT-Se对293T人胚胎肾细胞和AML-12小鼠正常肝细胞在200μg/mL时细胞存活率仍高于82%,同时DNT-Se能够抑制HepG2细胞的增殖,在72 h时对HepG2细胞的IC_(50)值为42.54μg/mL。本研究成功确定了DNT-Se的最优制备工艺,所得纳米硒粒径较小、分散均匀,对肝癌细胞HepG2有抑制增殖的效果,对正常细胞无毒性,具有良好的生物安全性。This study aimed to explore the optimal conditions for the preparation ofβ-glucan/nanosized selenium(Se NPs)complexes(DNT-Se)and determining the safety of DNT-Se.The concentration of polysaccharides(DNTs),the ratio of vitamin C(V_(C))to Na_(2)SeO_(3),the concentration of Na_(2)SeO_(3),the reaction time,and the reaction temperature were used as experimental factors.The dual-wavelength colorimetric method and the Malvern particle-size assay method were used to characterize changes in the particle size of Se NPs in DNT-Se,and the single-factors and orthogonal experimental design were used to investigate the preparation process of DNT-Se,and the toxicity of DNT-Se to 293T human embryonic kidney cells and normal liver cells of AML-12 mice was evaluated by using CCK-8.The results showed that the optimal conditions for DNT-Se preparation were list as follows:1.0 mg/mL of DNTs,and 1.0 mg/mL of Na_(2)SeO_(3) with the ratio of vitamin C to Na_(2)SeO_(3) 6:1.Moreover,the reaction time was 12 h,and the reaction temperature was 25℃.The mean diameter of DNT-Se prepared under such conditions was around 34.50 nm.Cell proliferation experiment results showed that DNT-Se was non-toxic to 293T human embryonic kidney cells and normal liver cells of AML-12 mice,but it could inhibit the proliferation of HepG2 cells,and the IC_(50) value of DNT-Se for HepG2 cells at 72 h was 42.54μg/mL.The synthesis process of DNT-Se was fixed in this work,and the obtained nanoparticles were with smaller size and uniform distribution.Moreover,the DNT-Se could significantly inhibit the proliferation of HepG2 hepatoma cells without toxicity to normal cells,which was of good biosafety.

关 键 词：纳米硒 黑木耳 Β-葡聚糖 工艺考察 

分 类 号：R943[医药卫生—药剂学]