复合酶-超声波辅助提取火龙果花黄酮及抗氧化性研究  

Study on the Extraction of Flavonoids from Pitaya Flower by Complex Enzyme-ultrasound and Its Antioxidant Activity

作　　者：张中华 徐西俊 陈凤真[2] 阮帝 李霞[2] 何盈 ZHANG Zhonghua;XU Xijun;CHEN Fengzhen;RUAN Di;LI Xia;HE Ying(Heze Agricultural Technology Extension Center,Heze 274000,China;College of Agricultural and Biological Engineering,Heze University,Heze 274000,China)

机构地区：[1]菏泽市农业技术推广中心,山东菏泽274000 [2]菏泽学院农业与生物工程学院,山东菏泽274000

出　　处：《中国果菜》2023年第10期29-35,共7页China Fruit & Vegetable

基　　金：山东省高等学校黄河流域高效生态特色植物资源产业化开发协同创新中心(2022);菏泽市生殖营养功能重点实验室(2022)。

摘　　要：火龙果花富含营养元素和生物活性成分,本文采用复合酶-超声波辅助提取火龙果花黄酮,通过单因素结合响应面试验,优化提取工艺条件,并测定火龙果花黄酮抗氧化性。结果表明,最佳提取工艺条件为液料比48∶1(mL/g),乙醇体积分数80%,酶解时间63 min,火龙果花黄酮的提取得率为10.28%;当浓度为1.2 mg/mL时,火龙果花黄酮对ABTS^(+)·和超氧阴离子的清除率分别为45.65%和39.67%,说明火龙果花中的黄酮类化合物抗氧化能力较强。此研究能为火龙果花的综合利用提供参考。Pitaya flower is rich in nutrients and bioactive ingredients.In this paper,compound enzyme-ultrasound assisted extraction of flavonoids from pitaya flower was used.Through single factor experiments and Plackett-Burman analysis,the factors that significantly affected the extraction rate of flavonoids from pitaya flower were liquid-solid ratio,ethanol volume fraction and enzymatic hydrolysis time.Then the response surface optimization was used to determine the best extraction conditions,and the antioxidant experiment was carried out.The highest yield of flavonoids(10.28%)was obtained at liquid-to-material ratio of 48∶1(mL/g),the volume fraction of ethanol with 80%,63 min of enzymatic hydrolysis time.When the concentration was 1.2 mg/mL,the removal rates of ABTS^(+)·and superoxide anions by the flavonoid extract were 45.65%and 39.67%,respectively,which indicated that the flavonoids from pitaya flowers had strong antioxidant capacity and could be used as natural antioxidants to increase comprehensive utilization.

关 键 词：火龙果花 复合酶-超声波 黄酮 抗氧化性 

分 类 号：TS209[轻工技术与工程—食品科学]