Divergent chondro/osteogenic transduction laws of fibrocartilage stem cell drive temporomandibular joint osteoarthritis in growing mice  

作　　者：Ruiye Bi Qianli Li Haohan Li Peng Wang Han Fang Xianni Yang Yiru Wang Yi Hou Binbin Ying Songsong Zhu 

机构地区：[1]State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&Department of Orthognathic and TMJ Surgery,West China Hospital of Stomatology,Sichuan University,Chengdu,China [2]State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&West China Hospital of Stomatology,Sichuan University,Chengdu,China [3]Department of Stomatology,Ningbo First Hospital,Ningbo,China

出　　处：《International Journal of Oral Science》2023年第3期462-475,共14页国际口腔科学杂志（英文版）

基　　金：supported by the National Natural Science Foundation of China(NSFC)No.82071139(to S.Z.),81873720(to B.Y.),82270999(to R.B.);Key R&D Program of Sichuan Provincial Department of Science and Technology No.23ZDYF2130(to S.Z.);Sichuan Science and Technology Program No.2022NSFSC1382(to Y.H.);‘From Zero to One’Innovative Research Program of Sichuan University No.2022SCUH0022(to R.B.);Clinical Research Program of West China Hospital of Stomatology,LCYJ2023-DL-5。

摘　　要：The anterior disc displacement(ADD)leads to temporomandibular joint osteoarthritis(TMJOA)and mandibular growth retardation in adolescents.To investigate the potential functional role of fibrocartilage stem cells(FCSCs)during the process,a surgical ADDTMJOA mouse model was established.From 1 week after model generation,ADD mice exhibited aggravated mandibular growth retardation with osteoarthritis(OA)-like joint cartilage degeneration,manifesting with impaired chondrogenic differentiation and loss of subchondral bone homeostasis.Lineage tracing using Gli1^(-)CreER^(+);Tm^(fl/-)mice and Sox9-CreER^(+);Tm^(fl/-)mice showed that ADD interfered with the chondrogenic capacity of Gli1+FCSCs as well as osteogenic differentiation of Sox9+lineage,mainly in the middle zone of TMJ cartilage.Then,a surgically induced disc reposition(DR)mouse model was generated.The inhibited FCSCs capacity was significantly alleviated by DR treatment in ADD mice.And both the ADD mice and adolescent ADD patients had significantly relieved OA phenotype and improved condylar growth after DR treatment.In conclusion,ADD-TMJOA leads to impaired chondrogenic progenitor capacity and osteogenesis differentiation of FCSCs lineage,resulting in cartilage degeneration and loss of subchondral bone homeostasis,finally causing TMJ growth retardation.DR at an early stage could significantly alleviate cartilage degeneration and restore TMJ cartilage growth potential.

关 键 词：CARTILAGE impaired DEGENERATION 

分 类 号：R782.6[医药卫生—口腔医学]