opaque2对糯玉米子粒食味和营养品质的影响分析  

Effect of opaque2 on the Taste and Nutritional Quality of Waxy Corn Kernels

作　　者：刘晓丽 韩洁楠 李冉 郭增辉 张德贵[1] 李明顺[1] LIU Xiao-li;HAN Jie-nan;LI Ran;GUO Zeng-hui;ZHANG De-gui;LI Ming-shun(Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081;College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163319,China)

机构地区：[1]中国农业科学院作物科学研究所,北京100081 [2]黑龙江八一农垦大学农学院,黑龙江大庆163319

出　　处：《玉米科学》2023年第4期52-58,共7页Journal of Maize Sciences

基　　金：国家玉米产业技术体系项目(CAR02-01);中国农业科学院创新工程项目“主要农产品品质与营养调控研究”(CAAS-XTCX20190025-2)。

摘　　要：玉米opaque2(o2)是胚乳发育的核心调控基因,对子粒发育至关重要。基于9组糯玉米o2近等基因系(o2-NILs)及对应轮回亲本,对鲜食期和成熟期子粒的种皮厚度、总淀粉含量、可溶性糖含量等性状进行分析。结果表明,黄糯2/o2o2和中63-2/o2o2鲜食期子粒的食味品质性状均有提高,其他o2-NILs子粒品质变化不一。鲜食期o2突变对种皮厚度影响较大,但与成熟期无直接相关性。o2突变使玉米子粒赖氨酸含量增加,营养价值提高,但多数近等基因系成熟期淀粉含量和百粒重降低,仅糯2/o2o2、淀粉含量和百粒重均不受影响。糯2/o2o2、SY1-2/o2o2、赵OP-6/o2o2中27 KDγ-zein表达量显著增加、子粒饱满度与轮回亲本相近,但均表现为粉质胚乳,因此认为,优质蛋白糯玉米硬质胚乳的恢复需多个o2胚乳修饰基因协同作用。opaque2(o2)is a core regulatory gene for endosperm development in corn and is essential for kernels development.The traits such as seed coat thickness,total starch content,and soluble sugar content in fresh and ma-ture kernels were analyzed based on nine near-isogenic lines(o2-NILs)and their corresponding recurrent parents.The results indicated that the taste quality of Huangnuo2/0202 and Zhong63-2/0202 were improved at fresh stage,while the taste quality of other o2-NILs varied differently.o2 mutation had a great effect on seed coat thickness at fresh eating stage,but it had no direct correlation with that at mature stage.o2 mutation has increased lysine content and improved nutritional value of corn kernels in o2-NILs,but most genotypes have lower starch content and 100-grain weight at maturity,except Nuo2/0202.The 27 KDγ-zein in Nuo2/0202,SY1-2/0202 and Zhao0P-6/0202 in-creased significantly,and the kernel fullness was similar to that of recurrent parent,but the kernels still exhibited an opaque endosperm.Therefore,the recovery of hard endosperm requires cooperation of multiple o2 endosperm modi-fication genes.

关 键 词：糯玉米 opaque2 食味品质 营养品质 

分 类 号：S513.024[农业科学—作物学]