Tripterygium hypoglaucum extract ameliorates adjuvant-induced arthritis in mice through the gut microbiota  

作　　者：HU Jianghui NI Jimin ZHENG Junping GUO Yanlei YANG Yong YE Cheng SUN Xiongjie XIA Hui LIU Yanju LIU Hongtao 

机构地区：[1]College of Basic Medical Sciences,Hubei University of Chinese Medicine,Wuhan 430065,China [2]Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China [3]Wuhan Customs Technology Center,Wuhan 430050,China

出　　处：《Chinese Journal of Natural Medicines》2023年第10期730-744,共15页中国天然药物（英文版）

基　　金：supported by the Health Commission of Hubei Province of China(No.ZY2021Z005);the Major Science and Technology Project in Yunnan Province(No.202102AE090042)。

摘　　要：Traditionally,Tripterygium hypoglaucum(Levl.)Hutch(THH)are widely used in Chinese folk to treat rheumatoid arthritis(RA).This study aimed to investigate whether the anti-RA effect of THH is related with the gut microbiota.The main components of prepared THH extract were identified by HPLC-MS.C57BL/6 mice with adjuvant-induced arthritis(AIA)were treated with THH extract by gavage for one month.THH extract significantly alleviated swollen ankle,joint cavity exudation,and articular cartilage destruction in AIA mice.The mRNA and protein levels of inflammatory mediators in muscles and plasma indicated that THH extract attenuated inflammatory responses in the joint by blocking TLR4/MyD88/MAPK signaling pathways.THH extract remarkably restored the dysbiosis of the gut microbiota in AIA mice,featuring the increases of Bifidobacterium,Akkermansia,and Lactobacillus and the decreases of Butyricimonas,Parabacteroides,and Anaeroplasma.Furthermore,the altered bacteria were closely correlated with physiological indices and drove metabolic changes of the intestinal microbiota.In addition,antibiotic-induced pseudo germ-free mice were employed to verify the role of the intestinal flora.Strikingly,THH treatment failed to ameliorate the arthritis symptoms and signaling pathways in pseudo germ-free mice,which validates the indispensable role of the intestinal flora.For the first time,we demonstrated that THH extract protects joint inflammation by manipulating the intestinal flora and regulating the TLR4/MyD88/MAPK signaling pathway.Therefore,THH extract may serve as a microbial modulator to recover RA in clincial practice.verRAinclincial practice.

关 键 词：ARTHRITIS Gut microbiota IMMUNITY INFLAMMATION Tripterygium hypoglaucum 

分 类 号：R965[医药卫生—药理学]