织纹螺肉中河豚毒素基体标准物质研制及不确定度评定  

Development and uncertainty evaluation of matrix certified reference material for tetrodotoxin in Nassarius meat

作　　者：王兴龙 诸寅 杨大进 任一平 WANG Xinglong;ZHU Yin;YANG Dajin;REN Yiping(Yangtze Delta Region Research Institute of Tsinghua University,Jiaxing 314000,China;China National Center for Food Safety Risk Assessment,Beijing 100050,China)

机构地区：[1]浙江清华长三角研究院,嘉兴314000 [2]国家食品安全风险评估中心,北京100050

出　　处：《分析试验室》2023年第9期1163-1169,共7页Chinese Journal of Analysis Laboratory

基　　金：国家重点研发计划(2017YFC1601302);浙江省公益研究计划(LGC20B020002)项目资助。

摘　　要：研制以织纹螺肉为基体的河豚毒素标准物质。9家专业实验室采用液相色谱-串联质谱(LC-MS/MS)外标法对织纹螺肉中河豚毒素的含量进行联合定值。该基体标准物质特性值为(0.78±0.14)mg/kg,组间与组内均匀性良好。在4℃冷藏运输条件下,短期稳定性可达14 d。在-18℃冷冻条件下,长期稳定性可达12个月。该标准物质测量结果的不确定度主要来源于均匀性、稳定性及定值过程。本标准物质适用于水产品中河豚毒素的分析方法验证和质量控制。A matrix certified reference material(CRM)for tetrodotoxin(TTX)in Nassarius meat was developed.LC-MS/MS with external standard method was employed by nine expert laboratories for the characterization of TTX content in Nassarius meat.The certified value of CRM was(0.78±0.14)mg/kg.The CRM was homogeneous for TTX between and within bottles.The short-term stability of the CRM at 4℃ for transportation was up to 14 days and the long-term stability at -18℃ storage condition was up to 12 months.The uncertainty was assessed by combing the contributions from homogeneity,stability and characterization.This CRM would be applicable to the analytical method validation and quality control in the determination of tetrodotoxin in Nassarius meat.

关 键 词：液相色谱-串联质谱法 织纹螺 河豚毒素 有证标准物质 定值 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]