Anti-hepatic carcinoma mechanisms of calycosin through targeting ferroptosis  

作　　者：Litao Nie Yimei Liao Rui Zhou Xiao Liang Xiaowei Wan Xin Li Min Su 

机构地区：[1]Key Laboratory of Environmental Pollution and Integrative Omics,Guilin Medical University,Guilin,Guangxi Zhuang Autonomous Region 541004,China [2]Department of Clinical Pharmacy,Guigang City People's Hospital,The Eighth Affiliated Hospital of Guangxi Medical University,Guigang,Guangxi Zhuang Autonomous Region 537100,China [3]Department of Hepatobiliary Surgery,Guigang City People's Hospital,The Eighth Affiliated Hospital of Guangxi Medical University,Guigang,Guangxi Zhuang Autonomous Region 537199,China [4]Guangxi Key Laboratory of Tumor Immunology and Microenvironmental Regulation,Guilin Medical University,Guilin,Guangxi Zhuang Autonomous Region 541004,China

出　　处：《Intelligent Medicine》2023年第3期173-179,共7页智慧医学(英文)

基　　金：supported by the National Natural Science Foundation of Guangxi (Grant No.2020GXNSFBA159066).

摘　　要：Background Ferroptosis,a pathologic state induced by lipid-driven oxidative stress,is associated with the development of human cancers.Calycosin,a natural compound with antioxidant and anti-inflammatory activities,has promising antitumor effects.However,the ferroptosis-related mechanism of calycosin in the treatment of hepatic carcinoma has not been reported.Methods This study applied network pharmacology and bioinformatic approaches(including Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis)to investigate the pharmacologic targets and mechanism of action of calycosin in the treatment of hepatic carcinoma through targeting ferroptosis.By searching online databases including The Cancer Genome Atlas,FerrDb,GeneCards,SwissTargetPrediction,SuperPred,BindingDB,TargetNet,BATMAN-TCM,and Drugbank,we identified 13 ferroptosis-related putative target genes of calycosin against hepatic carcinoma including IL-6,PTGS2,SRC,HRAS,NQO1,NOX4,PGK1,G6PD,GPI,MIF,NOS2,ALDOA,and SQSTM1.Results Molecular docking analysis revealed that calycosin potentially binded directly with the target proteins IL-6,PTGS2,and SRC.Functional enrichment analysis of these proteins indicated that they were involved in gluconeogenesis and apoptosis through regulation of ERK1,ERK2,and MAPK activities(P<0.05).Conclusion Calycosin exerts antitumor effects in hepatic carcinoma by targeting ferroptosis through regulation of IL-6,PTGS2,and SRC.

关 键 词：Hepatic carcinoma Ferroptosis CALYCOSIN BIOINFORMATICS Pharmacologic target 

分 类 号：R735.7[医药卫生—肿瘤]