酶解法制备鹿血生物活性肽的工艺研究  

Study on the preparation of bioactive peptides from deer blood by enzymatic hydrolysis

作　　者：高倩倩 付慧 李红莹 王英臣 GAO Qian-qian;FU Hui;LI Hong-ying;WANG Ying-chen(College of Food Engineering,Jilin Agricultural Science and Technology College,Jilin 132101,Jilin,China;National Food Nutrition Education and Demo Base,Brewing Technology Innovation Center of Jilin province,Jilin Province Engineering Research Center for Higher Education on Brewing Technology,Jilin 132101,Jilin,China)

机构地区：[1]吉林农业科技学院食品工程学院,吉林吉林132101 [2]国家食物营养教育示范基地,吉林省酿造技术科技创新中心,酿造技术吉林省高等学校工程研究中心,吉林吉林132101

出　　处：《粮食与油脂》2023年第11期95-99,共5页Cereals & Oils

基　　金：2022年吉林省教育厅科学技术研究项目(JJKH20220392KJ);2022年大学生科技创新项目(SJ2022063)。

摘　　要：以鹿血为原料,采用超声波破碎法制备血红蛋白粉,然后对其进行酶解制备鹿血生物活性肽。在筛选出最适蛋白酶的基础上,以DPPH自由基的清除率为指标,通过单因素试验结合响应面法优化鹿血生物活性肽的酶解法制备工艺。结果表明:最适蛋白酶为木瓜蛋白酶;最佳制备工艺为酶解温度58℃、酶解时间4 h、酶添加量7.3%(以血红蛋白粉质量计)、pH 10.2,在此条件下,鹿血生物活性肽对DPPH自由基的清除率最大,为75.96%。Using deer blood as raw material,the hemoglobin powder was prepared by ultrasonic crushing method,and then the deer blood bioactive peptide was prepared by enzymolysis.On the basis of selecting the most suitable protease,taking DPPH free radical clearance rate as index,the enzymatic preparation process of deer blood bioactive peptide was optimized by single factor test and response surface method.The results showed that the most suitable protease was papain.The optimal preparation process was enzy-matic hydrolysis temperature 58℃,enzymatic hydrolysistime4h,enzymeadditionamount7.3%(based on the mass of hemoglobin powder),pH 10.2.Under these conditions,the deer blood bioactive peptide had the highest clearance rate of DPPH free radicals,which was 75.96%.

关 键 词：鹿血 生物活性肽 蛋白酶 血红蛋白粉 

分 类 号：TS201.21[轻工技术与工程—食品科学]