超声协助吐温-20一步法制备水分散性虾青素工艺探究及其表征  

One-step Preparation and Characterization of Water-dispersible Astaxanthin Emulsion by Tween-20 under Ultrasonic Condition

作　　者：李佳 齐祥明[1,2,3] LI Jia;QI Xiangming(Qingdao Key Laboratory of Food Biotechnology,College of Food Science and Engineering,Ocean University of China,Qingdao 266404,China;Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China;Key Laboratory of Biological Processing of Aquatic Products,China National Light Industry,Qingdao 266404,China)

机构地区：[1]中国海洋大学食品科学与工程学院,青岛市食品生物技术重点实验室,山东青岛266404 [2]青岛海洋科学与技术试点国家实验室海洋药物与生物制品功能实验室,山东青岛266237 [3]中国轻工业水产品生物加工重点实验室,山东青岛266404

出　　处：《食品工业科技》2023年第22期182-190,共9页Science and Technology of Food Industry

基　　金：山东省重点研发项目(2019JZZY010814)。

摘　　要：为同时实现雨生红球藻中虾青素的绿色提取和性质改良,本文采用超声协助吐温-20同步实现了对其中虾青素的高效释放和水分散性虾青素纳米乳液的制备,进一步探究了该过程的影响因素和水分散性虾青素纳米乳液的形成机理,并对所得水分散虾青素进行基本成分分析和初步的性能表征。工艺探索结果表明:在显著影响因素吐温-20添加量、超声功率、料液比分别为200μL、600 W、1:20 g/mL时,获得了高达98.41%的虾青素分散率。机理探究结果显示:超声处理具有实现雨生红球藻有限破胞和充分协助吐温-20分散虾青素的双重作用,比高压均质更具工艺优越性。产品表征结果显示:该条件下所获得的水分散虾青素乳液颗粒平均粒径为115.55 nm,Zeta电位为-23.35 mV;虾青素包封量高达产品干重的43.82%,且全部为无定形非晶态;被选择性包封的脂质中饱和脂肪酸量偏高,占干重的17.93%。总的来说,本文所开发的超声协助吐温-20一步法制备水分散虾青素乳液工艺可直接从雨生红球藻中获得虾青素产品,操作简便、提取高效、绿色可行,且产品部分性能优于纯虾青素包封产品。In order to achieve sustainable extraction and enhance the properties of astaxanthin from Haematococcus pluvialis,a green and efficient approach was developed by utilizing Tween-20 under ultrasonic conditions.This approach allowed for the efficient release of astaxanthin while simultaneously preparing water-dispersible astaxanthin emulsions.The factors influencing the process,the formation mechanism of the water-dispersible astaxanthin emulsion,and the composition and performance characterization of the obtained product were investigated.Exploration of the process revealed that Tween-20 dosage,ultrasonic power,and solid-to-liquid ratio significantly influenced the release and dispersion efficiency of astaxanthin,with 98.41%astaxanthin being released and dispersed when Tween-20 dosage,ultrasonic power,and solid-to-liquid ratio were 200μL,600 W,and 1:20 g/mL,respectively.The mechanism exploration results demonstrated that ultrasound had a dual effect of disrupting the cells of H.pluvialis to a limited extent and assisting Tween-20 to disperse astaxanthin,which was superior to high-pressure homogenization.The characterization results showed that the water-dispersible astaxanthin emulsion obtained under these conditions had an average particle size of 115.55 nm,a Zeta potential of-23.35 mV,and an encapsulated astaxanthin content of up to 43.82%in the dry product.Astaxanthin was found to be encapsulated in an amorphous non-crystalline state,with selective encapsulation of saturated fatty acids.The amount of saturated fatty acids was up to 17.93%(wt.%).Overall,this one-step,ultrasonic approach to preparing water-dispersible astaxanthin emulsions from H.pluvialis using Tween-20 is easy to operate,highly efficient,green and feasible,with some of the product properties being superior to those of pure astaxanthin encapsulated products.

关 键 词：虾青素 超声处理 释放 吐温-20 纳米乳液 成分分析 性能表征 

分 类 号：TS201.1[轻工技术与工程—食品科学]