Urine biomarkers discovery by metabolomics and machine learning for Parkinson’s disease diagnoses  

作　　者：Xiaoxiao Wang Xinran Hao Jie Yan Ji Xu Dandan Hu Fenfen Ji Ting Zeng Fuyue Wang Bolun Wang Jiacheng Fang Jing Ji Hemi Luan Yanjun Hong Yanhao Zhang Jinyao Chen Min Li Zhu Yang Doudou Zhang Wenlan Liu Xiaodong Cai Zongwei Cai 

机构地区：[1]State Key Laboratory of Environmental and Biological Analysis,Department of Chemistry,Hong Kong Baptist University,Hong Kong,China [2]Department of Neurosurgery,Shenzhen Key Laboratory of Neurosurgery,the First Affiliated Hospital of Shenzhen University,Shenzhen Second People’s Hospital,Shenzhen 518035,China [3]The Central Laboratory,the First Affiliated Hospital of Shenzhen University,Shenzhen Second People’s Hospital,Shenzhen 518035,China [4]Department of Nutrition,Food Safety and Toxicology,West China School of Public Health,Sichuan University,Chengdu 610041,China [5]Mr.and Mrs.Ko Chi Ming Centre for Parkinson’s Disease Research,School of Chinese Medicine,Hong Kong Baptist University,Hong Kong,China

出　　处：《Chinese Chemical Letters》2023年第10期93-97,共5页中国化学快报（英文版）

基　　金：support from the Collaborative Research Fund(No.C2011–21GF);from Guangdong Province Basic and Applied Basic Research Foundation(No.2021B1515120051).

摘　　要：Parkinson’s disease(PD)is a complex neurological disorder that typically worsens with age.A wide range of pathologies makes PD a very heterogeneous condition,and there are currently no reliable diagnostic tests for this disease.The application of metabolomics to the study of PD has the potential to identify disease biomarkers through the systematic evaluation of metabolites.In this study,urine metabolic profiles of 215 urine samples from 104 PD patients and 111 healthy individuals were assessed based on liquid chromatography-mass spectrometry.The urine metabolic profile was first evaluated with partial leastsquares discriminant analysis,and then we integrated the metabolomic data with ensemble machine learning techniques using the voting strategy to achieve better predictive performance.A combination of 8-metabolite predictive panel performed well with an accuracy of over 90.7%.Compared to control subjects,PD patients had higher levels of 3-methoxytyramine,N-acetyl-l-tyrosine,orotic acid,uric acid,vanillic acid,and xanthine,and lower levels of 3,3-dimethylglutaric acid and imidazolelactic acid in their urine.The multi-metabolite prediction model developed in this study can serve as an initial point for future clinical studies.

关 键 词：Parkinson’s disease High-resolution mass spectrometry BIOMARKER METABOLOMIC Machine learning 

分 类 号：R742.5[医药卫生—神经病学与精神病学] TP181[医药卫生—临床医学]