动物双歧杆菌乳亚种V9连续传代过程中遗传稳定性评价  

Evaluation of Genetic Stability during Continuous Passage of the Bifidobacterium animals subsp.lactis V9

作　　者：刘凯龙 黄天 刘晓晔 玉霞 姚国强[1] Liu Kailong;Huang Tian;Liu Xiaoye;Yu Xia;Yao Guoqiang(Key Laboratory of Dairy Biotechnology and Engineering of Ministry of Education,Key Laboratory of Dairy Processing of Ministry of Agriculture and Rural Affairs,Inner Mongolia Agricultural University,Key Laboratory of Dairy Biotechnology and Engineering of Inner Mongolia Autonomous Region,Huhhot 010018)

机构地区：[1]内蒙古农业大学、乳品生物技术与工程教育部重点实验室、农业农村部奶制品加工重点实验室、内蒙古自治区乳品生物技术与工程重点实验室,呼和浩特010018

出　　处：《中国食品学报》2023年第9期12-22,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS36);内蒙古自治区高等学校科学技术研究项目(NJZZ22488);内蒙古自治区高层次人才科研支持经费项目(2021)。

摘　　要：目的:以具有优良益生特性的动物双歧杆菌乳亚种V9为研究对象,对其连续传代过程中的遗传稳定性进行研究。方法:动物双歧杆菌乳亚种V9在TPY培养基中连续培养100代,对其0,25,50,75,100代的菌体形态、碳水化合物利用情况以及结合比较基因组学进行综合分析。结果:动物双歧杆菌乳亚种V9在连续传代过程中不同代时的菌体形态、碳水化合物利用情况均无显著差异(P>0.05),并以动物双歧杆菌乳亚种V9原始基因组作为参考序列,研究发现不同代时的动物双歧杆菌乳亚种V9的基因组大小和GC含量与原始基因组均无显著差异(P>0.05),系统发育树结果表明不同代时的动物双歧杆菌乳亚种V9与原始基因组亲缘关系较近,通过识别SNP突变位点发现不同代时的动物双歧杆菌乳亚种V9基因组保守,均未发现稳定遗传的突变位点,进一步进行碳水化合物代谢活性酶注释,结果发现不同代时菌株具有相似的碳水化合物代谢相关基因,遗传特征高度相似。结论:本研究从表型特性和基因组层面综合评估了动物双歧杆菌乳亚种V9的遗传稳定性,可为其进一步开发研究及产业化提供保障。Objective:In this study,the genetic stability of Bifidobacterium animalis subsp.lactis V9 with excellent prebiotic properties was used as the study object to study its genetic stability in continuous passage.Methods:When the Bifidobacterium animalis subsp.lactis V9 was continuously transmitted in TPY medium for 100 generations,the colony morphology,carbohydrate utilization and combined comparative genomics of 0,25,50,75 and 100 generations were comprehensively analyzed.Results:There were no significant differences in phenotypic characteristics such as bacterial morphology and carbohydrate utilization of Bifidobacterium animalis subsp.lactis V9 during the continuous passage process(P>0.05),And with the original genome of the Bifidobacterium animalis subsp.lactis V9 as a reference sequence,The study found that the genome size and GC content of the Bifidobacterium animalis subsp.lactis V9 at different generations were not significantly different from the original genome(P>0.05),and the phylogenetic tree results showed that the Bifidobacterium animalis subsp.lactis V9 at different generations was closely related to the original genome,and by identifying the SNP mutation site,it was found that the genome of the Bifidobacterium animalis subsp.lactis V9 at different generations was conservative,and no stable genetic mutation sites were found,and further carbohydrate metabolism active enzyme annotation was carried out,and it was found that the strains at different generations had similar carbohydrate metabolism-related genes,and the genetic characteristics were highly similar.Conclusion:The Bifidobacterium animalis subsp.lactis V9 has excellent genetic stability during continuous passage,which provides a theoretical basis for the further development and industrialization of probiotics.

关 键 词：动物双歧杆菌乳亚种V9 连续传代 遗传稳定性 

分 类 号：TS201.3[轻工技术与工程—食品科学]