清香大曲中高耐受性功能酵母的筛选  

Screening of high tolerance functional yeasts from light-flavor Daqu

作　　者：牟飞燕 夏博宇 张维 董孝元 陈晖[3] 常煦[3] 陈茂彬 方尚玲 MOU Feiyan;XIA Boyu;ZHANG Wei;DONG Xiaoyuan;CHEN Hui;CHANG Xu;CHEN Maobin;FANG Shangling(Key Laboratory of Fermentation Engineering(Ministry of Education),Hubei Key Laboratory of Industrial Microbiology,Hubei Province Cooperative Innovation Center of Industrial Fermentation,School of Biological Engineering and Food,Hubei University of Technology,Wuhan 430068,China;Yellow Crane Tower Wine Industry Co.,Ltd.,Wuhan 430068;Angel Yeast Co.,Ltd.,Yichang 443000,China)

机构地区：[1]湖北工业大学生物工程与食品学院工业发酵省部共建协同创新中心湖北省工业微生物重点实验室发酵工程教育部重点实验室,湖北武汉430068 [2]黄鹤楼酒业有限公司,湖北武汉430068 [3]安琪酵母股份有限公司,湖北宜昌443000

出　　处：《中国酿造》2023年第11期163-168,共6页China Brewing

基　　金：“十三五”国家重点研发计划重点专项(2016YFD0400500);湖北工业大学研究生教学改革研究项目资助(校2021057)。

摘　　要：以清香型大曲为研究对象,采用稀释涂布平板法从中分离酵母菌,对其形态特征进行观察,采用2,3,5-氯化三苯基四氮唑(TTC)显色法初筛,通过发酵力、产酒及产香能力分析进行复筛。对筛选酵母菌进行分子生物学鉴定及耐高温、耐乙醇、耐高糖、耐酸能力检测,筛选出耐受性优良的功能性酵母菌。结果表明,从清香型大曲中共分离初筛22株酵母菌,复筛后得到4株功能酵母菌,菌株B-6、C-3、D-10、D-11分别被鉴定为费比恩塞伯林德纳氏酵母(Cyberlindnera fabianii)、酿酒酵母(Saccharomyces cerevisiae)、扣囊复膜孢酵母(Saccharomycopsis fibuligera)、异常威克汉姆酵母(Wickerhamomyces anomalus)。耐受性分析结果表明,菌株B-6和C-3可耐受39℃、pH值为3、酒精度12%vol、葡萄糖质量浓度400 g/L环境条件。因此,菌株B-6和C-3可作为潜在功能菌株应用于白酒生产中。The yeasts were isolated from the light-flavor Daqu using the dilution-coated plate method,and their morphological characteristics were observed.Initially,the yeasts were screened using the 2,3,5-triphenyltetrazolium chloride(TTC)color development method,and rescreened by analysis of fermentation power,alcohol production and aroma production capacity.The screened yeasts were identified by molecular biology and tested for high temperature tolerance,ethanol tolerance,high sugar tolerance and acid tolerance,and the functional yeasts with good tolerance were screened.The results revealed the isolation of a total of 22 yeast strains from the light-flavor Daqu during the primary screening.After rescreening,four functional yeast strains were obtained,with strains B-6,C-3,D-10,and D-11 identified as Cyberlindnera fabianii,Saccharomyces cerevisiae,Saccharomycopsis fibuligera and Wickerhamomyces anomalus,respectively.Tolerance analysis demonstrated that strains B-6 and C-3 exhibited resilience to environmental conditions of temperature 39℃,pH 3,alcohol content 12%vol and glucose mass concentration 400 g/L.Therefore,strains B-6 and C-3 could be used as potential functional strains in Baijiu production.

关 键 词：清香型大曲 功能酵母菌 筛选 耐受性 

分 类 号：TS261.1[轻工技术与工程—发酵工程]