肉桂醛熏蒸对金黄色葡萄球菌胞内生物大分子的影响  

Effect of Cinnamaldehyde Fumigation on Intracellular Biological Macromolecules of Staphylococcus aureus

作　　者：段雪娟[1] 黄煜强 张潼 韩雅莉 吴克刚[1] 黄庶识[3] Duan Xuejuan;Huang Yuqiang;Zhang Tong;Han Yali;Wu Kegang;Huang Shushi(School of Chemical Engineering and Light Industry,Guangdong University of Technology,Guangzhou 510006;School of Biomedicine and Pharmaceutical Sciences,Guangdong University of Technology,Guangzhou 510006;Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry,Guangxi Academy of Sciences,Nanning 530007)

机构地区：[1]广东工业大学轻工化工学院,广州510006 [2]广东工业大学生物医药学院,广州510006 [3]广西科学院广西海洋天然产物与组合生物合成化学重点实验室,南宁530007

出　　处：《中国食品学报》2023年第10期90-100,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：广东省林业科技创新重点项目(2020KJCX010);广东省农村科技特派员重点项目(19ZK0364);广州市科技计划项目(202103000078)。

摘　　要：为研究肉桂醛熏蒸对金黄色葡萄球菌的影响,通过平板熏蒸法和菌块转移法研究其抗菌活性,通过透射电镜观察肉桂醛对菌体形态的影响,通过激光镊子拉曼光谱分析肉桂醛熏蒸对金黄色葡萄球菌胞内生物大分子的影响。结果表明,肉桂醛熏蒸对金黄色葡萄球菌具有良好的抗菌活性,最低抑菌浓度(MIC)和最低杀菌浓度(MBC)分别为0.25μL/mL和0.5μL/mL。经肉桂醛气熏后的菌体表面皱缩,可能与高浓度肉桂醛导致胞内物质外泄有关。激光镊子拉曼结果表明,肉桂醛气熏处理导致菌体蛋白主链、氢键体系以及空间结构发生改变,DNA单、双链发生断裂,脂酰基链骨架断裂或交联,同时,菌体脂双层的有序度也发生改变,影响膜的流动性,从而菌体的生长受到抑制或致其死亡。激光镊子拉曼光谱可以通过分析拉曼位移和峰强变化,分析单个细胞内生物大分子的骨架和结构变化,从而推断抗菌物质的抗菌机理。The antibacterial activity of vapour-phase cinnamaldehyde fumigation against Staphylococcus aureus(S.aureus)was studied by plate fumigation method and colony transfer method.The effect of cinnamaldehyde on bacterial morphology and intracellular biomacromolecules was investigated by transmission electron microscopy(TEM)and laser tweezers Raman spectroscopy(LTRS),respectively.The results showed that cinnamaldehyde had good gas-phase antibacterial activity against S.aureus,with the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of 0.25μL/mL and 0.5μL/mL,respectively.After fumigating with cinnamaldehyde,the surface of the cells shrank.The high concentration of cinnamaldehyde may lead to the leakage of intracellular substances.The results of LTRS showed that the content of intracellular biomacromolecules increased when cinnamaldehyde was below the MBC,but decreased when cinnamaldehyde was above the MBC.With cinnamaldehyde treatment,the main chain,hydrogen bond system and spatial structure of bacterial proteins were changed,DNA single and double strands were broken,fatty acyl chain backbones were broken or cross-linked,and the order of the body lipid bilayer also changed,which affecting the fluidity of the membrane,as a result,the bacteria was inhibited in growth or killed.The results of this research showed that LTRS could detect the changes of biomacromolecules in bacteria at the single-cell level.The skeleton and structural changes of biomacromolecules in a single cell can be analyzed to infer antibacterial substances by analyzing the Raman shift and peak intensity changes.It provides a new method for studying the antibacterial mechanism of antibacterial substances on single cells.

关 键 词：激光镊子拉曼光谱 肉桂醛 金黄色葡萄球菌 抗菌活性 机理 

分 类 号：TS201.3[轻工技术与工程—食品科学]