改性SiOx/WPU涂膜对腐败希瓦氏菌生物被膜的抑制作用  

Inhibition Mechanism of Modified SiOx/WPU Coatings on Biofilm of Shewanella putrefaciens Bacteria

作　　者：杨峻乙 张家涛 刘亮军 李秀霞 王莉丽 杨旭 孙彤 励建荣 Yang Junyi;Zhang Jiatao;Liu Liangjun;Li Xiuxia;Wang Lili;Yang Xu;Sun Tong;Li Jianrong(College of Food Science and Technology,Collaborative Innovation Center of Seafood Deep Processing,Bohai University,Jinzhou 121013,Liaoning;College of Food Science and Technology,Inner Mongolia Agricultural University,Hohhot 010018;Minze Longyangsxia Aquaculture Co.,Ltd.,Hainan Tibetan Autonomous Prefecture 811800,Qinghai)

机构地区：[1]渤海大学食品科学与工程学院海洋食品精深加工关键技术省部共建协同创新中心,辽宁锦州121013 [2]内蒙古农业大学食品科学与工程学院,呼和浩特010018 [3]民泽龙羊峡生态水殖有限公司,青海海南藏族自治州811800

出　　处：《中国食品学报》2023年第10期216-228,共13页Journal of Chinese Institute Of Food Science and Technology

基　　金：辽宁省教育厅科研项目(LJKMZ20221488);国家重点研发计划蓝色粮仓科技创新重点专项(2019YFD0901702)。

摘　　要：为获得对水产品中腐败希瓦氏菌生物被膜具有优良抑制性能的水性聚氨酯(WPU)涂膜,以不同微观形貌的SiOx微纳米粒子为粗糙度构建因子,采用滴涂法制备SiOx/WPU涂膜,测定改性SiOx/WPU涂膜表面的疏水疏油性能、表面能、微观形貌和热稳定性能,揭示改性SiOx/WPU涂膜对腐败希瓦氏菌生物被膜的抑制机制。结果表明,以十六烷基三甲基溴化铵(CTAB)改性的SiOx颗粒为粗糙度构建因子制备的C-SiOx/WPU涂膜,尽管表面氟含量最高,但其水和正十六烷接触角分别为139.0°±3.5°和0°,仅表现为疏水超亲油性,热稳定性和抗细菌黏附性最差;改性气相纳米SiOx/WPU涂膜表现为超疏水超亲油性,热稳定性最好,且可以抑制腐败希瓦氏菌的初期黏附;以改进的Stober法在同一溶剂体系中制备和改性SiOx微纳米粒子为粗糙度构建因子制备的改性微米级SiOx/WPU涂膜为超双疏表面,热稳定性较高,对腐败希瓦氏菌不可逆黏附的抑制最好,可有效降低生物被膜的代谢活性,减少胞外多糖(EPS)的分泌,培养24 h后其表面刚开始形成微菌落。本研究的超双疏性改性微米级SiOx/WPU涂膜可用于制备食品包装材料,为抗生物被膜材料在食品包装领域的应用提供技术支持。In order to obtain waterborne polyurethane(WPU)coatings with excellent inhibition properties against Shewanella putrefaciens bacteria biofilm in aquatic products,SiOx/WPU coatings were prepared by drop coating method with SiOx nanoparticles of different micromorphology as roughness construction factors.The hydrophobicity and oleophobic,surface energy,surface morphology and thermal stability of the modified SiOx/WPU coatings were measured.The inhibition mechanism of the modified SiOx/WPU coatings on the Shewanella putrefaciens biofilm was revealed.The results show that,using SiOx particles modified by cetyltrimethylammonium bromide(CTAB)as the roughness construction factor,the C-SiOx/WPU coating surface has the highest fluorine content,but its contact angles of water and n-hexadecane are 139.0°±3.5°and 0°,respectively,and shows hydrophobic and super-lipophilic,prepared by using SiOx particles modified by and has the worst thermal stability and anti-bacterial adhesion.The modified fumed nano-SiOx/WPU coating is super-hydrophobic and super-lipophilic,which has the highest thermal stability and could inhibit the initial adhesion of Shewanella putrefaciens.The modified micron scale SiOx/WPU coating was prepared by improved Stober method and modified SiOx micro-nano particles in the same solvent system as the roughness construction factor.The modified micron scale SiOx/WPU coating was super-amphiphobic surface with high thermal stability,which could effectively inhibit the irreversible adhesion of Shewanella putrefaciens,reduce its metabolic activity and EPS secretion,and microcolonies just began to form on its surface,after 24 h of culture.The super-amphiphobic modified micron scale SiOx/WPU coatings in this study can be used to prepare food packaging materials,providing technical support for the application of anti-biofilm materials in the field of food packaging.

关 键 词：SIOX 水性聚氨酯 超双疏 涂膜 腐败希瓦氏菌 生物被膜 

分 类 号：TS201.3[轻工技术与工程—食品科学]