兴安升麻醇提物成分分析及基于网络药理学探讨其促进皮肤伤口愈合的作用机制  

Analysis of the Components of Cimicifuga dahurica(Turcz.)Maxim.Alcohol Extract and Exploration of Its Mechanism of Promoting Skin Wound Healing Based on Network Pharmacology

作　　者：李艳娜 张富源 程伟峰 桑亚新[1] 王向红[1] LI Yanna;ZHANG Fuyuan;CHENG Weifeng;SANG Yaxin;WANG Xianghong(College of Food Science and Technology,Hebei Agricultural University,Baoding 071000,China;Hebei Yaguo Food Co.,Ltd.,Baoding 071000,China)

机构地区：[1]河北农业大学食品科技学院,河北保定071000 [2]河北雅果食品有限公司,河北保定071000

出　　处：《食品工业科技》2023年第24期12-22,共11页Science and Technology of Food Industry

基　　金：河北省现代农业产业技术体系蔬菜产业创新团队计划(HBCT2018030208);科技部“一带一路”创新人才交流外国专家项目(DL2021003001)。

摘　　要：为了深入挖掘兴安升麻生理功能,本研究采用醇提法提取活性成分,通过HPLC-MS分析兴安升麻的化学成分并基于网络药理学探讨其对皮肤伤口的促愈机制。结果表明,兴安升麻中鉴定出125种化学成分,其中萜类、苯丙素类及酮类化合物含量较多,主要包括隐绿原酸、绿原酸、白术内脂Ⅲ等活性成分,可作用于一个或多个靶点,EGFR、KDR、F2可能为促愈过程中发挥作用的重要靶点,且橘皮素、山姜素、5-去甲川陈皮素等活性成分可能在此过程中发挥重要作用,说明兴安升麻具有多成分、多靶点作用于促进皮肤伤口愈合的特性;兴安升麻可能通过VEGF、EGFR和TNF信号通路调控相关因子和蛋白的表达,发挥对皮肤伤口的促愈作用。本研究探明了兴安升麻醇提物的主要活性成分,并基于网络药理学预测了促进皮肤伤口愈合的作用机制,为后续兴安升麻的开发利用提供试验及理论基础。In order to further explore the physiological functions of Cimicifuga dahurica(Turcz.)Maxim.,the study extracted the active compounds by alcohol extraction method,analyzed the chemical compositions by HPLC-MS,and explored its healing mechanism on skin wounds based on network pharmacology.The results showed that 125 chemical components were identified from Cimicifuga dahurica(Turcz.)Maxim.,and the contents of terpenoids,phenylpropanoids and ketones were higher,including cryptochlorogenic acid,chlorogenic acid,atractylenolide Ⅲ and others.The active compounds of Cimicifuga dahurica(Turcz.)Maxim.could regulate one or more targets.The EGFR,KDR,F2 genes might be important targets in the process of promoting healing,and tangeretin,alpinetin,and 5-O-demethylnobiletin and other active compounds might also play important roles in this process.It was proved that Cimicifuga dahurica(Turcz.)Maxim.had a characteristic of multi-component and multi-target action to promote skin wound healing.After the action of Cimicifuga dahurica(Turcz.)Maxim.,the expression of related factors and proteins might be regulated through VEGF,EGFR,and TNF signaling pathways,exerting a promoting effect on skin wound healing.Through the above research,the main active ingredients of Cimicifuga dahurica(Turcz.)Maxim.alcohol extract were identified,and the mechanism of promoting skin wound healing was predicted based on network pharmacology,which would provide the experimental and theoretical basis for the subsequent development and utilization of Cimicifuga dahurica(Turcz.)Maxim.

关 键 词：兴安升麻 化学成分 促进皮肤伤口愈合 网络药理学 作用机制 

分 类 号：TS201[轻工技术与工程—食品科学]