Inhibition of VEGF-A expression in hypoxia-exposed fetal retinal microvascular endothelial cells by exosomes derived from human umbilical cord mesenchymal stem cells  

作　　者：JING LI WANWAN FAN LILI HAO YONGSHENG LI GUOCHENG YU WEI SUN XIANQIONG LUO JINGXIANG ZHONG 

机构地区：[1]Department of Ophthalmology,The First Affiliated Hospital of Jinan University,Jinan University,Guangzhou,510630,China [2]Department of Ophthalmology,Guangdong Women and Children Hospital,Guangzhou,511400,China [3]Department of Ophthalmology,The Sixth Affiliated Hospital of Jinan University,Jinan University,Dongguan,523000,China [4]Graduate School,Guangzhou Medical University,Guangzhou,510000,China [5]Lab Center,Guangdong Cord Blood Bank,Guangzhou,510000,China [6]Guangdong Eye Institute,Department of Ophthalmology,Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences),Southern Medical University,Guangzhou,510000,China

出　　处：《BIOCELL》2023年第11期2485-2494,共10页生物细胞（英文）

基　　金：supported by the following funds:1.Medical Scientific Research Foundation of Guangdong Province(A2022221);Natural Science Foundation of Guangdong Province(2019A1515011417);National Natural Science Foundation of China(81970806,82271094);Science and Technology Projects in Guangzhou(202201020030,202201020015);Guangdong High-Level Hospital Construction Fund(ynkt2021-zz16).

摘　　要：Objective:This study aimed to investigate the potential of human umbilical cord mesenchymal stem cell(hucMSC)-derived exosomes(hucMSC-Exos)in inhibiting hypoxia-induced cell hyper proliferation and overexpression of vascular endothelial growth factor A(VEGF-A)in immature human fetal retinal microvascular endothelial cells(hfRMECs).Methods:Exosomes were isolated from hucMSCs using cryogenic ultracentrifugation and characterized through various techniques,including transmission electron microscopy,nanoparticle tracking analysis,bicinchoninic acid assays,and western blotting.The hfRMECs were identified using von Willebrand factor(vWF)co-staining and divided into four groups:a control group cultured under normoxic condition,a hypoxic model group,a hypoxic group treated with low-concentration hucMSC-Exos(75μg/mL)and a hypoxic group treated with high-concentration hucMSC-Exos(100μg/mL).Cell viability and proliferation were assessed using Cell Counting Kit-8(CCK-8)assay and EdU(5-ethynyl-2′-deoxyuridine)assay respectively.Expression levels of VEGF-A were evaluated using RT-PCR,western blotting and immunofluorescence.Results:Hypoxia significantly increased hfRMECs’viability and proliferation by upregulating VEGF-A levels.The administration of hucMSC-Exos effectively reversed this response,with the high-concentration group exhibiting greater efficacy compared to the lowconcentration group.Conclusion:In conclusion,hucMSC-Exos can dose-dependently inhibit hypoxia-induced hyperproliferation and VEGF-A overexpression in immature fetal retinal microvascular endothelial cells.

关 键 词：Mesenchymal stem cells EXOSOMES Immature fetal retinal vascular endothelial cells Vascular endothelial growth factor A HYPOXIA 

分 类 号：R774.1[医药卫生—眼科]