复合酶解提取牡蛎糖胺聚糖及其降血糖活性  

Study on Extraction of Glycosaminoglycan from Oyster by Complex Enzymatic Hydrolysis and Hypoglycemic Activity

作　　者：王雅茹 陈晖 阎光宇 林河通[1] 洪专 WANG Yaru;CHEN Hui;YAN Guangyu;LIN Hetong;HONG Zhuan(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350001,Fujian,China;Technology Innovation Center for Exploitation of Marine Biological Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,Fujian,China;Xiamen Ocean Vocational College,Xiamen 361022,Fujian,China)

机构地区：[1]福建农林大学食品科学学院,福建福州350001 [2]自然资源部第三海洋研究所海洋生物资源开发利用工程技术创新中心,福建厦门361005 [3]厦门海洋职业技术学院,福建厦门361022

出　　处：《食品研究与开发》2023年第23期105-113,共9页Food Research and Development

基　　金：福建省科技计划项目(2020N0029);厦门市科技计划项目(3502Z20221036)。

摘　　要：以福建牡蛎为原料,采用单因素试验和响应面法优化试验确定牡蛎糖胺聚糖复合酶解提取的工艺条件,并对其α-葡萄糖苷酶抑制活性进行研究。结果显示,当料液比为1∶15(g/mL)、起始pH8.0、复合酶添加量2.20%、复合酶质量比(中性蛋白酶∶胰蛋白酶)1∶1.20、酶解温度50℃、酶解时间4 h时,可以达到对牡蛎糖胺聚糖最佳的提取效果,提取率为(0.71±0.02)%。体外α-葡萄糖苷酶的抑制结果显示,牡蛎糖胺聚糖对α-葡萄糖苷酶的抑制作用在0.2~1.0 mg/mL时呈正相关,当牡蛎糖胺聚糖样品浓度为1.0 mg/mL时,对α-葡萄糖苷酶抑制率可达到42.65%,表明其具有一定的降血糖功效。The response surface method was used to optimize the extraction conditions of glycosaminoglycan compound enzyme from Fujian oyster,and theα-glucosidase inhibitory activity of oyster glycosaminoglycan was studied.The results showed that when the solid-liquid ratio was 1∶15(g/mL),initial pH8.0,compound enzyme dosage 2.20%,mass ratio of complex enzyme(neutral protease∶trypsin)1∶1.20,enzymolysis temperature 50℃,enzymolysis time 4 h,the optimal extraction effect of oyster glycosaminoglycan was obtained,and the extraction rate reached(0.71±0.02)%.The inhibition results ofα-glucosidase in vitro showed that the inhibitory effect of oyster glucosaminoglycan onα-glucosidase was positively correlated in the range of 0.2-1.0 mg/mL.When the sample concentration of oyster glucosaminoglycan was 1.0 mg/mL,the inhibition rate ofα-glucosidase could reach 42.65%,indicating that oyster glucosaminoglycan had certain hypoglycemic effect.

关 键 词：牡蛎 糖胺聚糖 复合酶解法 降血糖 α-葡萄糖苷酶抑制活性 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]