响应面法优化麦冬黄酮纯化工艺及其对运动疲劳的影响  

Optimization on purification technology of flavonoids from Ophiopogon japonicus by response surface methodology and its effect on exercise fatigue

作　　者：田洪霞[1] 牛生洋[1] TIAN Hong-xia;NIU Sheng-yang(Henan Institute of Science and Technology,Xinxiang 453003,Henan,China)

机构地区：[1]河南科技学院,河南新乡453003

出　　处：《粮食与油脂》2023年第12期84-88,93,共6页Cereals & Oils

基　　金：河南省自然科学基金面上项目(202300410164)。

摘　　要：以麦冬黄酮的吸附率和解吸率为考察指标,探讨HPD-300大孔树脂纯化麦冬黄酮的最佳工艺条件,并通过小鼠的运动疲劳模型,比较不同产物缓解运动疲劳的活性。结果表明:最佳纯化条件为上样液质量浓度0.4 mg/mL、上样液pH4.1、上样流速0.9 mL/min、上样体积50 mL、洗脱液体积100 mL(体积分数70%乙醇溶液)和洗脱流速1.9 mL/min,所得产物的总黄酮含量由18.2%±0.5%提高至69.4%±1.2%。与空白对照组和提取组相比,灌胃高剂量(0.4 g/kg·d)麦冬黄酮纯化物的小鼠游泳力竭时间显著延长(P<0.05),同时运动后血清中乳酸与尿素氮的含量显著下降(P<0.05)。Using the adsorption and desorption rates of flavonoids from Ophiopogon japonicus as indica-tors,the optimal process conditions for purifying flavonoids from Ophiopogon japonicus using HPD-300 macroporous resin were explored.The activity of different products in alleviating exercise fatigue was compared using a mouse exercise fatigue model.The results showed that the optimal purification condi-tions were as follows:sample solution mass concentration 0.4 mg/mL,sample solution pH 4.1,flow rate 0.9 mL/min,sample volume 50 mL,eluent volume 100 mL(volume fraction 70%ethanol solution),and flow rate 1.9 mL/min.The total flavonoid content of the obtained product increased from 18.2%±0.5%to 69.4%±1.2%.Compared with blank control group and extraction group,the swimming ex-haustion time of mice administrated with high dose(0.4 g/kg·d)of flavonoids purified from Ophiopogon japonicus was significantly extended(P<0.05),and the contents of lactic acid and urea nitrogen in ser-um were significantly decreased after exercise(P<0.05).

关 键 词：麦冬 黄酮 大孔树脂 纯化 运动性疲劳 

分 类 号：TS201.2[轻工技术与工程—食品科学]