葡萄籽提取物复合膜制备及其抑菌性研究  

Research on the preparation and antibacterial activity of composite films of grape seed extract

作　　者：耿直[1] 付佳乐[1] 郭婉婷 GENG Zhi;FU Jia-le;GUO Wan-ting(School of Medicine,Shaanxi Institute of International Trade&Commerce,Xi’an 712046,China)

机构地区：[1]陕西国际商贸学院医药学院,西安712046

出　　处：《湖北农业科学》2023年第11期118-124,130,共8页Hubei Agricultural Sciences

基　　金：陕西省中药管理局课题(2021-ZZ-ZY007);陕西省大学生创新创业训练计划项目(S202113123012)。

摘　　要：以壳聚糖和海藻酸钠为成膜材料,以甘油为增塑剂,通过单因素试验,分别考察壳聚糖、海藻酸钠、甘油浓度和葡萄籽提取物添加量对复合膜抗拉伸强度和断裂伸长率的影响,在此基础上建立正交试验进行研究,采用K-B法测定葡萄籽提取物复合膜对大肠杆菌、沙门氏菌、铜绿假单胞菌和金黄色葡萄球菌的抗菌活性。结果表明,复合膜最佳成膜工艺为海藻酸钠浓度2.00%、壳聚糖浓度1.50%、甘油浓度0.2%、葡萄籽提取物20%。复合膜对4种控制菌均有抗菌活性,其中对大肠杆菌效果最好,其抑菌圈直径可达7.4 mm,对铜绿假单胞菌的抑菌效果最差。本试验方法简便迅速,所得复合膜稳定,具有一定的力学性能、适宜的透明度和吸水性,加入葡萄籽提取物使复合膜增加抗菌性能,为抗菌复合膜的制备提供一个新的思路,同时为食品保鲜行业膜类产品的制备提供借鉴和参考。Chitosan and sodium alginate were used as film forming materials and glycerin as the plasticizer.The effects of chitosan,so⁃dium alginate,glycerin concentration and grape seed extract on tensile strength and elongation at the break of composite films were in⁃vestigated by the single factor test,and the orthogonal test was established on this basis.The antibacterial activity of the composite films of grape seed extract against Escherichia coli,Salmonella,Pseudomonas aeruginosa and Staphylococcus aureus was determined by the K-B method.The results showed that the best composite films forming process was sodium alginate concentration of 2.00%,chi⁃tosan concentration of 1.50%,glycerin concentration of 0.2%,and grape seed extract of 20%.The composite films had antibacterial activity against all four control bacteria,with the best effect against Escherichia coli,its antibacterial sphere diameter of 7.4 mm and the worst antibacterial effect against Pseudomonas aeruginosa.The test method was simple and rapid,and the composite films were sta⁃ble,with certain mechanical properties,appropriate transparency and water absorption.Adding grape seed extract could increase the antibacterial properties of the composite films,which provided a new idea for the preparation of antibacterial composite films and pro⁃vided reference for the preparation of film products in the food preservation industry.

关 键 词：葡萄籽提取物 壳聚糖 海藻酸钠 复合膜 抑菌 

分 类 号：TS206.4[轻工技术与工程—食品科学]