miR-642a-3p对藻蓝色素抑制非小细胞肺癌活性的调控作用  

The Regulation Function of miR-642a-3p on the Inhibitory Activity of Non-small Cell Lung Cancer Mediated by Phycocyanin

作　　者：李前程 郝帅 李爽[1] 刘媛璞 李凡念 杨起 张文静[1] Li Qiancheng;Hao Shuai;Li Shuang;Liu Yuanpu;Li Fannian;Yang Qi;Zhang Wenjing(Key Laboratory of Geriatric Nutrition and Health(Beijing Technology and Business University),Ministry of Education,Beijing Engineering and Technology Research Center of Food Additives,School of Food and Health,Beijing Technology and Business Uniwversity,Beijing 100048)

机构地区：[1]老年营养与健康教育部重点实验室(北京工商大学),北京市食品添加剂工程技术研究中心,北京工商大学食品与健康学院,北京100048

出　　处：《中国食品学报》2023年第11期13-23,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金面上项目(32072231,32272321);北京市属高等学校优秀青年人才培育计划项目(BPHR202203042)。

摘　　要：藻蓝色素(PC)是一种来自海洋藻类的天然色素蛋白复合体,也是一类重要的食品功能因子,具有抗肿瘤、抗炎、增强免疫等生理活性,被广泛应用于功能性食品、医药及化妆品等领域。藻蓝色素对非小细胞肺癌(NSCLC)细胞活性的抑制作用已被证实,然而其机制尚不明确。本研究以miR-642a-3p为切入点,探究藻蓝色素抑制NSCLC细胞活性的机制。试验结果表明,藻蓝色素能够上调4种NSCLC细胞(A549、H1299、H460和LTEP-a2)中miR-642a-3p的表达水平。过表达干预细胞中的miR-642a-3p水平能够显著抑制4种细胞的体外增殖、迁移和非锚定生长能力。此外,转染miRNA抑制剂干预miR-642a-3p的表达,在一定程度上增强了细胞的活性。本研究初步揭示miR-642a-3p上调表达参与藻蓝色素抑制NSCLC细胞活性的过程,为藻蓝色素的深入研究和利用提高科学依据。Phycocyanin(PC)is a kind of natural pigment protein complex derived from marine algae.It is also a type of important food functional factor with anti-cancer,anti-inflammatory,and immune-enhancing activities,and has been widely used in functional food,medicine and cosmetics.Phycocyanin has been proven to inhibit the activity of non-small cell lung cancer(NSCLC)cells,but the underlying regulatory mechanism is not fully understood.This study focused on miR-642a-3p,and investigated the potential regulating mechanism of phycocyanin in NSCLC cells.The results showed that phycocyanin upregulated the expression level of miR-642a-3p in four NSCLC cells(A549,H1299,H460 and LTEP-a2).Overexpression of miR-642a-3p levels significantly inhibited the proliferation,migration and unanchored growth of four cells in vitro.In addition,transfection of miRNA inhibitors to interfere with the expression of miR-642a-3p could enhance cell viability to a certain extent.This study preliminarily revealed that the up-regulation expression of miR-642a-3p was involved in the regulation of inhibiting NSCLC cell viabilities by phycocyanin,which could lay a scientific theoretical basis for the in-depth study and utilization of phycocyanin.

关 键 词：藻蓝色素 miR-642a-3p 非小细胞肺癌 细胞活性 

分 类 号：TS201.4[轻工技术与工程—食品科学] R734.2[轻工技术与工程—食品科学与工程]