不同制剂对虾青素体外生物利用度的影响  

Effect of different delivery systems on the bioavailability of astaxanthin in vitro

作　　者：范超 宿惊涛 文瑾 李倩 吴文忠 FAN Chao;SU Jingtao;WEN Jin;LI Qian;WU Wenzhong(INNOBIO,Dalian 116600,China)

机构地区：[1]大连医诺生物股份有限公司,辽宁大连116600

出　　处：《食品与发酵工业》2023年第23期181-186,共6页Food and Fermentation Industries

摘　　要：虾青素作为一种脂溶性色素,因其低溶解度、低稳定性等原因,生物利用度较低。该研究旨在探究不同制剂类型的虾青素在体外消化吸收模拟中的影响情况。该实验采用体外胃肠道消化模型,考察虾青素油、微囊粉、微粒、乳液剂型的释放度,并将体外消化得到的各制剂终产物给予Caco-2细胞单层模型,考察各制剂消化终产物的表观渗透系数(apparent permeability values,P_(app)),对比不同剂型虾青素的体外生物利用度。在虾青素各制剂体外消化中,除油剂型外,其他剂型在模拟消化液中可以形成稳定的分散体系,油、微囊粉、微粒、乳液释放度分别为14.73%、93.71%、89.07%、67.11%,在Caco-2细胞单层模型中的Papp分别为0.058×10^(-6)、0.483×10^(-6)、0.461×10^(-6)、0.656×10^(-6)cm/s。虾青素的微囊粉、微粒、乳液制剂使虾青素的释放度及透过率均显著提升,有效提高了虾青素的生物利用度。As a lipid-soluble pigment,astaxanthin has low bioavailability due to its low solubility and low stability.This study aims to explore the effects of different formulation types of astaxanthin in in vitro digestion and absorption simulation model.A gastrointestinal digestion model in vitro was used to investigate the gastrointestinal release of astaxanthin oleoresin,microencapsulation powder,beadlets,and emulsion formulations.The final digestion products were further applied to the Caco-2 cell monolayer model to investigate the apparent permeability values(P_(app)),and to compare the bioavailability of different formulations of astaxanthin in vitro.In the digestion of various astaxanthin preparations in vitro,all dosage forms can form stable dispersion systems in the simulated digestive juice except for the oleoresin dosage form.The release rates of oleoresin,microencapsulation powder,beadlets,and emulsion were 14.73%,93.71%,89.07%,and 67.11%,respectively.The Papp in the Caco-2 cell monolayer model is 0.058×10^(-6),0.483×10^(-6),0.461×10^(-6),and 0.656×10^(-6) cm/s,respectively.Astaxanthin’s microencapsulation powder,beadlets,and emulsion formulations significantly increased the release and penetration of astaxanthin,which effectively improved the bioavailability of astaxanthin.

关 键 词：虾青素 微囊 生物利用度 体外消化 表观渗透系数 

分 类 号：TS264.4[轻工技术与工程—发酵工程]