超高效液相色谱-串联质谱法测定功能饮料中的肌醇、牛磺酸和咖啡因  

Determination of inositol,taurine and caffeine in functional drinks by ultra-high performance liquid chromatography-tandem mass spectrometry

作　　者：梁静 冯家力[1] 曾栋[1] 陈东洋[1] 刘先军[1] LIANG Jing;FENG Jiali;ZENG Dong;CHEN Dongyang;LIU Xianjun(Hunan Provincial Center for Disease Control and Prevention,Changsha,Hunan 410005,China)

机构地区：[1]湖南省疾病预防控制中心,湖南长沙410005

出　　处：《实用预防医学》2023年第11期1317-1320,共4页Practical Preventive Medicine

基　　金：湖南省自然科学基金(2021JJ70085);湖南省卫生健康委员会科研项目(B20160073,C20190035,D202312069311)。

摘　　要：目的建立一种同时检测功能饮料中肌醇、牛磺酸与咖啡因含量的超高效液相色谱-串联质谱法。方法功能饮料涡旋混匀后,移取0.02 ml,用超纯水稀释至10.0 ml,用0.22μm的滤膜过滤后,经Waters HSS T3色谱柱实现分离,流动相分别是甲醇和纯水,梯度洗脱,最佳流速是0.2 ml/min,通过三重四极杆质谱仪进行分析,外标法定量。结果分析物在0.005~1.0μg/ml范围内具有良好的线性,相关系数r≥0.9995;肌醇、牛磺酸与咖啡因的检出限依次为5、0.2和0.002 ng/ml;三种分析物的加标回收率在88.1%~109.0%,相对标准偏差(n=6)为0.8%~7.9%。结论该方法前处理简单、高效、准确、灵敏,适合同时分析功能饮料中的肌醇、牛磺酸与咖啡因。Objective To develop a method based on ultra-high performance liquid chromatography-tandem mass spectrometry for the simultaneous determination of inositol,taurine and caffeine in functional drinks.Methods After vortex mixing of functional drinks,0.02 ml mixture was diluted to 10.0 ml with ultra-pure water,and filtered through 0.22μm filter membrane.The samples were separated by Waters HSS T3 column,methanol-water as the mobile phases under gradient elution,and the optimal flow rate was 0.22 ml/min.The samples were determined by triple quadrupole mass spectrometry,and quantified by external standard method.Results The analytes had good linearity in the range of 0.005-1.0μg/ml,and the correlation coefficients(r)were greater than or equal to 0.9995.The detection limits of inositol,taurine and caffeine were 5 ng/ml,0.2 ng/ml and 0.002 ng/ml respectively.The recoveries of the three analytes were 88.1%-109.0%,and the relative standard deviations(RSD,n=6)0.8%-7.9%.Conclusion The method is simple for preprocessing,efficient,accurate and sensitive,and it is suitable for the simultaneous analysis of inositol,taurine and caffeine in functional drinks.

关 键 词：肌醇 牛磺酸 咖啡因 功能饮料 超高效液相色谱-串联质谱法 

分 类 号：O656.3[理学—分析化学]