桃金娘果实不溶性膳食纤维理化特征及其抗糖基化性能研究  

Physicochemical Characteristics and Antiglycation Capacity of Insoluble Dietary Fiber from Rhodomyrtus tomentosa Fruit

作　　者：邓叶俊 黄立新[1] 张彩虹[1] 谢普军[1] DENG Yejun;HUANG Lixin;ZHANG Caihong;XIE Pujun(Institute of Chemical Industry of Forest Products,CAF,Key Lab.of Biomass Energy and Material,Jiangsu Province,Key Lab.of Chemical Engineering of Forest Products,National Forestry and Grassland Administration,National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass,Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing 210042,China)

机构地区：[1]中国林业科学研究院林产化学工业研究所、江苏省生物质能源与材料重点实验室、国家林业和草原局林产化学工程重点实验室、林木生物质低碳高效利用国家工程研究中心、江苏省林业资源高效加工利用协同创新中心,江苏南京210042

出　　处：《林产化学与工业》2023年第6期43-50,共8页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金资助项目(32171732)。

摘　　要：分别采用水法和酶法改性提取制备桃金娘果实中不溶性膳食纤维(IDF),使用扫描电镜(SEM)、红外光谱(FT-IR)、X射线衍射(XRD)等方法表征其理化特性,并评估桃金娘IDF在蛋白糖基化各阶段抑制活性及其对晚期糖基化终末产物(AGE)的吸附性能。研究结果表明:水法提取IDF(WIDF)中水分、脂肪、蛋白质及灰分含量更高。酶法改性提取桃金娘IDF(EIDF)得率为67.43%,低于水法提取。2种方法制备的IDF官能团相似,但EIDF结晶度更低,为29.32%,EIDF的表面粗糙、结构松散,并伴有孔洞。当EIDF的质量浓度为2 g/L时,糖基化体系中果糖胺及羰基分别为(1.98±0.25)mmol/L和(21.75±1.17)μmol/g,β-淀粉样蛋白荧光强度(5.26±0.14)×10^(6),对AGE的抑制率为(18.15±0.73)%,EIDF抗糖基化能力优于WIDF。当EIDF质量浓度为10 g/L时,可吸附33.28%的AGE。The insoluble dietary fiber(IDF)in Rhodomyrtus tomentosa fruits was prepared by water method and enzymatic modification extraction,respectively.Scanning electron microscopy(SEM),Fourier transform infrared spectrometer(FT-IR),X-ray diffraction(XRD),and other methods were used to characterize their physicochemical characteristics.The inhibitory effects of R.tomentosa fruits IDF at each protein glycation stages as well as the adsorption capacity to advanced glycation end-products(AGE)were evaluated.The results indicate that the IDF prepared by water extraction method(WIDF)presented higher content of water,fat,protein and ash.The extraction yield of R.tomentosa fruits IDF by enzymatic modification extraction method(EIDF)was 67.43%,which was lower than that of water extraction method.The functional groups of IDF prepared by these two methods were similar,whereas the EIDF presented a lower comparative crystallinity at 29.32%.The rough surface,loose structure and holes were observed in EIDF.As the mass concentration of EIDF was 2 g/L,the contents of fructosamine and carbonyl compounds in glycation system were(1.98±0.25)mmol/L and(21.75±1.17)μmol/g,with fluorescence intensity ofβ-amyloid protein at(5.26±0.14)×106 and AGE inhibitory rate at(18.15±0.73)%,respectively,and the anti-glycation capacity of EIDF was better than that of WIDF.As the concentration of EIDF was 10 g/L,33.28%of AGE could be adsorbed.

关 键 词：膳食纤维 酶法改性 果糖胺 晚期糖基化终末产物 吸附 

分 类 号：TQ35[化学工程] TS202.1[轻工技术与工程—食品科学]