噬菌体受体结合蛋白及其宿主谱扩展的相关研究进展  

Progress in Research on Bacteriophage Receptor-Binding Proteins and Host Range Extension

作　　者：李凡 于振兴 张明 林洪[2] 李学鹏 励建荣 张德福 LI Fan;YU Zhenxing;ZHANG Ming;LIN Hong;LI Xuepeng;LI Jianrong;ZHANG Defu(National and Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,Institute of Ocean,College of Food Science and Engineering,Bohai University,Jinzhou 121013,China;College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China)

机构地区：[1]渤海大学食品科学与工程学院,海洋研究院,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州121013 [2]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品科学》2023年第23期252-260,共9页Food Science

基　　金：国家自然科学基金青年科学基金项目(31801662);“十三五”国家重点研发计划重点专项(2019YFD0901702)。

摘　　要：噬菌体是如今地球上种类最多、分布最广的生物之一,在清除细菌尤其是针对目前广泛出现的多重耐药细菌上具有巨大优势。目前多种噬菌体产品已在食品原料、养殖动物、食源性致病菌检测及即食食品杀菌等领域投入使用且效果良好,但天然噬菌体狭窄的宿主范围与复杂的宿主互作机制很大程度上限制了新型噬菌体抑菌剂的开发。噬菌体与宿主细菌的特异性结合依赖于噬菌体表面的受体结合蛋白,对各种不同的噬菌体受体结合蛋白的结构功能与作用机制进行研究有助于进一步了解噬菌体与细菌的互作过程,在此基础上利用诱导培养及基因工程等方法对噬菌体受体结合蛋白进行改造以获得优质的宽谱噬菌体已经成为噬菌体研究的重要内容之一。本文对噬菌体受体结合蛋白的不同结构与各种宿主谱扩展方案的相关研究进行总结,为食品工业中噬菌体的进一步开发与应用提供理论支撑。Phages are one of the most diverse and widely distributed organisms on the earth today,and have great advantages in eliminating bacteria,especially multi-drug resistant bacteria that are widespread at present.At present,a variety of bacteriophage products have been well applied in thefields of breeding for food raw materials,farmed animals,foodborne pathogenic bacteria detection,and instant food sterilization.However,the narrow host range and complex interaction mechanism between bacteriophage and host bacteria greatly limit their development as new bacteriostatic agents.The specific binding between bacteriophages and host bacteria depends on the receptor binding proteins on the surface of bacteriophages.Studying the structure,function and mechanism of action of various bacteriophage receptor binding proteins will help in understanding the interaction process between bacteriophage and bacteria.On this basis,phage receptor binding protein modification by genetic engineering and induced culture methods to obtain high-quality broad-spectrum phages has become an important content of phage research.This paper summarizes the current research on different structures of phage receptor binding proteins and various host spectrum expansion schemes in an effort to provide theoretical support for the development and application of bacteriophages in the food industry.

关 键 词：噬菌体 特异性结合 受体结合蛋白 基因改造 宿主谱扩展 食品领域 

分 类 号：TS201.3[轻工技术与工程—食品科学]