亚铁离子对罗非鱼片细菌生长的影响  

Influence of Ferrous Ions on Bacterial Growth on Tilapia Fillets

作　　者：孟晓华[1] MENG Xiaohua(Hebi Polytechnic,Hebi 458030,China)

机构地区：[1]鹤壁职业技术学院,河南鹤壁458030

出　　处：《肉类研究》2023年第10期16-21,共6页Meat Research

基　　金：粤港关键领域重点突破项目(2010A020104004)。

摘　　要：采用2 g/100 mL的氯化亚铁溶液处理罗非鱼片,定期取样测定鱼片的感官评分、菌落总数、挥发性盐基氮(total volatile basic nitrogen,TVB-N)和三甲胺(trimethylamine,TMA)含量,结合聚合酶链式反应-变性梯度凝胶电泳(polymerase chain reaction-denaturing gradient gel electrophoresis,PCR-DGGE)分析亚铁离子对鱼片上细菌生长的影响。结果表明,氯化亚铁处理鱼片的感官较好,菌落总数、TVB-N和TMA含量较对照鱼片明显降低,对照鱼片和氯化钠处理鱼片在4℃冷藏9 d,菌落总数、TVB-N和TMA含量超过国家标准规定限量,而氯化亚铁处理鱼片的菌落总数、TVB-N和TMA含量未超标。通过PCR-DGGE分析可知,氯化亚铁处理鱼片在冷藏初期的菌相复杂,冷藏6 d后,腐败希瓦氏菌(Shewanella putrefaciens)、嗜冷杆菌(Psychrobacter)数量增多,假单胞菌(Pseudomonas)数量减少,但Pseudomonas在整个菌相中依旧数量最多,仍是优势菌。After being treated with 2 g/100 mL ferrous chloride solution,tilapiafillets were sampled at regular intervals during storage for measurement of sensory score,total bacterial count,total volatile basic nitrogen(TVB-N)content,and trimethylamine(TMA)content.Using polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE),the effect of ferrous ions on bacterial growth on tilapiafillets was studied.Fillets treated with ferrous chloride solution had better sensory quality and significantly lower total bacterial count,TVB-N and TMA content compared to controlfillets treated with distilled water.After storage at 4℃for 9 days,the total bacterial count,TVB-N and TMA content of control samples and those treated with sodium chloride all exceeded the national limits,while those of ferrous chloride treatedfillets did not.PCR-DGGE analysis showed that the bacterialflora of ferrous chloride treatedfillets was complicated in the early stage of cold storage.After 6 days,the number of Shewanella putrefaciens and Psychrobacter increased,while the number of Pseudomonas decreased,but was still the largest in the whole microflora;it remained the dominant bacterium.

关 键 词：鱼片 亚铁离子 细菌 变性梯度凝胶电泳 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]