茯苓三萜对α-葡萄糖苷酶和α-淀粉酶的抑制作用及酶动力学研究  

The Inhibition Effects and Enzyme Kinetics of Poria Cocos Triterpenes onα-glucosidase andα-amylase

作　　者：高满军 黄兴琼 莫启贵 陈迷 赵宝清 张丹丹 GAO Man-jun;ZHAO Bao-qing;ZHANG Dan-dan(School of Pharmacy,Xianning Medical College,Hubei University of Science and Technology,Xianning Hubei 437100,China)

机构地区：[1]湖北科技学院医学部药学院,湖北咸宁437100 [2]湖北科技学院糖尿病心脑血管病变湖北省重点实验室

出　　处：《湖北科技学院学报（医学版）》2023年第6期465-469,共5页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：糖尿病心脑血管病变湖北省重点实验室开放基金项目(2022TNB05);湖北科技学院博士科研启动基金(BK202324)。

摘　　要：目的探讨不同浓度茯苓三萜对α-葡萄糖苷酶和α-淀粉酶的抑制活性,及其酶促反应动力学特性。方法采用体外α-葡萄糖苷酶和α-淀粉酶抑制活性筛选模型,测定不同浓度的茯苓三萜类对两种酶的抑制作用,采用Lineweaver-Burk双倒数法分析其酶动力学性质。结果体外酶活性抑制试验显示,不同浓度的茯苓三萜类在体外对α-葡萄糖苷酶和α-淀粉酶均有抑制作用,其最大半数抑制浓度(IC_(50))分别为1.40μg/mL、10.43μg/mL,茯苓三萜对α-葡萄糖苷酶的抑制作用为可逆非竞争性抑制,对α-淀粉酶的抑制作用为可逆混合抑制类型。结论茯苓三萜类物质有降血糖活性,且浓度越高抑制效果越明显。本研究可为茯苓降血糖药物的研究和开发提供科学依据。Objective To investigate the inhibition effects and kinetic properties of Poria cocos triterpenes onα-glucosidase andα-amylase.Methods The inhibitory activities of different concentrations of Poria cocos triterpene onα-glucosidase andα-amylase were tested in vitro,and the kinetic properties were analyzed by Lineweaver-Burk.Results The in vitro enzyme activity inhibition assay showed that different concentrations of Poria triterpenoids inhibited bothα-glucosidase andα-amylase in vitro,and their half-maximum inhibitory concentrations(IC_(50))were 1.40μg/mL and 10.43μg/mL,respectively,and the inhibitory effect of Poria triterpenoids was reversible and non-competitive inhibition onα-glucosidase,and the inhibitory effect onα-amylase was reversible mixed inhibition type.Conclusion The Poria cocos triterpenes have hypoglycemic activity,and show concentration-dependent manner.This study can provide a scientific basis for the research and development of Poria cocos hypoglycemic drugs.

关 键 词：茯苓三萜 Α-葡萄糖苷酶 Α-淀粉酶 降血糖 酶动力学 

分 类 号：R285[医药卫生—中药学]