产马乳酒乳杆菌马乳酒亚种ZW18益生基因分析  

Analysis of probiotic genes of Lactobacillus kefiranofaciens subsp.kefiranofaciens ZW18

作　　者：李欣媛 杨凤妍 吕厚姣 耿伟涛 王艳萍[1] LI Xinyuan;YANG Fengyan;LV Houjiao;GENG Weitao;WANG Yanping(College of Food Science and Engineering,Tianjin University of Science and Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学食品科学与工程学院,天津300457

出　　处：《中国酿造》2023年第12期62-69,共8页China Brewing

摘　　要：产马乳酒乳杆菌马乳酒亚种(Lactobacillus kefiranofaciens subsp.kefiranofaciens)作为新食品原料具有很多益生特性,该研究利用全基因组测序对产马乳酒乳杆菌马乳酒亚种ZW18基本特征和益生功能基因进行全面分析。结果表明,该菌株由一个环状染色体和两个质粒组成,全长约2.4 Mbp,有更多独特基因和特性。通过数据库注释可得菌株ZW18具有较好的生存能力与稳定性,且对碳水化合物利用能力强,保证了菌体细胞的物质基础和能量供给。该菌株基因组中含有与抵抗氧化应激、耐酸耐胆盐、黏附和聚集、免疫、细菌素等相关的基因,与硫胺素、核黄素等多种维生素及赖氨酸、蛋氨酸等人体所需氨基酸合成相关基因,表明其具有多种潜在益生特性,为进一步深入研究与应用提供基础数据。As a new food raw material,Lactobacillus kefiranofaciens subsp.kefiranofaciens has many probiotic properties,and the basic characteristics and probiotic functional genes of L.kefiranofaciens subsp.kefiranofaciens ZW18 were analyzed by whole genome sequencing.The results showed that the strain was composed of a circular chromosome and two plasmids with a total length of about 2.4 Mbp,had more unique genes and characteristics.According to the database annotation,the strain ZW18 had good survival ability and stability,and strong carbohydrates utilization ability,which ensured the material basis and energy supply of bacterial cells.The genome of the strain ZW18 contained genes related to oxidative stress resistance,acid resistance and bile salt resistance,adhesion and aggregation,immunity,bacteriocin,etc.,and genes related to various vitamins such as thiamine and riboflavin,and amino acids required by the human body such as lysine and methionine,indicating that it had a variety of potential probiotic properties,providing basic data for further research and application.

关 键 词：产马乳酒乳杆菌马乳酒亚种 基因组 益生特性 

分 类 号：TS201.3[轻工技术与工程—食品科学]