食源性椰毒假单胞菌及其外毒素检测方法研究进展  

Advancements in the Detection Methods of Food-Borne Pseudomonas Cocovenenans and Its Exotoxins

作　　者：李会杰 陈桂芳 高运华[1] 杨佳怡 董莲华[1] LI Huijie;CHEN Guifang;GAO Yunhua;YANG Jiayi;DONG Lianhua(Center for Advanced Measurement Science,National Institute of Metrology,Beijing 100029;Shenzhen Institute for Technology Innovation,National Institute of Metrology,Shenzhen 518107;College of Chemistry Engineering,Shenyang University of Chemistry Technology,Shenyang 110142)

机构地区：[1]中国计量科学研究院前沿计量科学中心,北京100029 [2]深圳中国计量科学研究院技术创新研究院,深圳518107 [3]沈阳化工大学化学工程学院,沈阳110142

出　　处：《计量科学与技术》2023年第9期25-31,共7页Metrology Science and Technology

基　　金：中国计量科学研究院基本科研业务费项目(AKYZZ2322、AKYZZ2023)。

摘　　要：椰毒假单胞菌是革兰氏阴性菌,多生长于谷类发酵制品、薯类制品、变质银耳和木耳等农产品表面,并可以产生具有毒性作用的米酵菌酸和毒黄素,影响农产品质量安全。该菌所产生的外毒素会作用于人体大脑、肾脏等实质性器官,对机体消化系统和神经系统等造成损伤,致死率较高,个别案例可达100%。简要阐述了椰毒假单胞菌致病机制及其分型方法,系统论述该菌及其外毒素的检测方法研究进展,展望未来的研究方向,以期为椰毒假单胞菌研究提供参考。Pseudomonas cocovenenans,a gram-negative bacterium,predominantly grows on the surface of agricultural products like fermented grains,potato products,spoiled Tremella fuciformis(white fungus),and Auricularia auricula(wood ear fungus).This bacterium produces bongkrekic acid and toxoflavin,toxic substances that compromise the quality and safety of agricultural products.Its exotoxins affect vital human organs such as the brain and kidneys,causing significant harm to the digestive and nervous systems,and in some cases,lead to fatal outcomes,occasionally reaching a 100%fatality rate in individual instances.This paper outlines the pathogenic mechanism and molecular typing methods of Pseudomonas cocovenenans and provides a systematic review of the research progress in detection methods for the bacterium and its exotoxins.Furthermore,the paper discusses future research directions,aiming to serve as a reference for ongoing studies on Pseudomonas cocovenenans.

关 键 词：计量学 椰毒假单胞菌 米酵菌酸 毒黄素 检测方法 食品 

分 类 号：TB99[一般工业技术—计量学]