昆明犬源乳酸菌的分离、鉴定与益生特性研究  

Isolation,Identification and Probiotic Characteristics of Lactic Acid Bacteria Derived from Kunming Dog

作　　者：李吉琴 展丽梅 自绍平 梅会友 高彦航 徐虎 徐乐 鲍国樱 李杰 曹振辉[1] LI Jiqin;ZHAN Limei;ZI Shaoping;MEI Huiyou;GAO Yanhang;XU Hu;XU Le;BAO Guoying;LI Jie;CAO Zhenhui(Collage of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Kunming Police Dog Base of the Ministry of Public Security,Kunming 650204,China)

机构地区：[1]云南农业大学动物科学技术学院,昆明650201 [2]公安部昆明警犬基地,昆明650204

出　　处：《动物营养学报》2023年第12期8133-8147,共15页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：云南省中青年学术和技术带头人后备人才(202305AC160040)。

摘　　要：本试验旨在筛选优质犬源乳酸菌,为犬用微生态制剂的研发奠定基础。以昆明犬新鲜粪便作为菌株分离来源,通过MRS+碳酸钙固体培养基分离纯化乳酸菌,经16S rRNA测序鉴定得到21株乳酸菌,测定并评价其抗生素敏感性、抑菌活性、胃肠道耐受性、黏附能力和抗炎能力,综合比较不同菌株之间的益生特性。结果表明:从昆明犬新鲜粪便中分离筛选出7株具有良好的抗生素敏感性、抑菌活性和胃肠道耐受性的乳酸菌菌株,经过16S rRNA基因序列分析,MKLQ3807-13、MKLQ3807-35、MKLQ4704-19、MKLQ4704-30、MKLQ4704-49和MKCH1884-30为罗伊氏乳杆菌,MKCH1884-43为格氏乳杆菌。MKLQ3807-13、MKLQ3807-35、MKLQ4704-19、MKLQ4704-30和MKCH1884-43对肠上皮细胞HT-29具有较强的黏附能力;MKLQ3807-13和MKCH1884-43可显著降低肿瘤坏死因子-α(TNF-α)诱导的HT-29细胞白细胞介素-8(IL-8)基因相对表达量,且具有较好的自聚集能力与表面疏水性。综上所述,MKLQ3807-13和MKCH1884-43这2株乳酸菌有优良的益生特性,可为警犬专用微生态制剂的研发奠定基础。This study aimed to isolate canine-derived lactic acid bacteria,which would provide a basis for the development of canine microecological preparations.Using fresh feces of Kunming dog as the source of strain isolation,and lactic acid bacteria were isolated and purified using MRS agar supplemented with calcium carbonate.A total of 21 strains of lactic acid bacteria were identified based on 16S rRNA sequencing.Their antibiotic sensitivity,antagonistic activity,gastrointestinal tolerance,adhesion ability and anti-inflammatory ability were determined and evaluated.The probiotic characteristics between different strains were compared comprehensively.The results showed that seven strains with good antibiotic sensitivity,bacteriostatic activity and gastrointestinal fluid tolerance were isolated and screened from the fresh feces of Kunming dogs,and MKLQ3807-13,MKLQ3807-35,MKLQ4704-19,MKLQ4704-30,MKLQ4704-49 and MKCH1884-30 were Lactobacillus reuteri and MKCH1884-43 was Lactobacillus gasseri.MKLQ3807-13,MKLQ3807-35,MKLQ4704-19,MKLQ4704-30 and MKCH1884-43 had strong adhesion ability to intestinal epithelial cells HT-29.MKLQ3807-13 and MKCH1884-43 could significantly decrease the relative expression level of proinflammatory gene interleukin-8(IL-8)in tumor necrosis factor-alpha(TNF-α)-induced HT-29 cells,and had good self-aggregation ability and hydrophobicity.In conclusion,the two lactic acid bacteria strains MKLQ3807-13 and MKCH1884-43 have excellent probiotic properties,which can lay a foundation for the development of microecological preparations for police dogs.

关 键 词：昆明犬 乳酸菌 分离鉴定 益生特性 

分 类 号：S829.2[农业科学—畜牧学]