羊乳酪蛋白2种不同膜分离方法比较研究  

Comparative analysis of two different membrane separation methods for goat milk casein

作　　者：李欣雨 孟毅 贺宝元 周妍 魏怡 连严慧 张磊[1] 土双静 薛海燕[1] LI Xinyu;MENG Yi;HE Baoyuan;ZHOU Yan;WEI Yi;LIAN Yanhui;ZHANG Lei;TU Shuangjing;XUE Haiyan(College of Food Science and Engineering,Shaanxi University of Science and Technology,Xi'an 710021,China;College of Light Industry Science and Engineering,Shaanxi University of Science and Technology,Xi'an 710021,China;Shaanxi Jinniu Dairy Co.Ltd.,Weinan 714000,China)

机构地区：[1]陕西科技大学食品科学与工程学院,西安710021 [2]陕西科技大学轻工科学与工程学院,西安710021 [3]陕西金牛乳业有限公司,陕西渭南714000

出　　处：《中国乳品工业》2023年第12期24-30,共7页China Dairy Industry

基　　金：陕西省重点研发项目(2020ZDLNY02-09);陕西省科技计划项目(2022GY-361,2022KXJ-011);陕西省教育厅服务地方专项项目(19JC05);西安市科技计划农业科技创新工程项目(20NYYF0016)。

摘　　要：为量化中空纤维素膜和卷式有机膜分离羊乳中酪蛋白和乳清蛋白的能力,在相同的操作压力和料液温度下,从渗透液的感官、SDS-PAGE定性、凯氏定氮的定量和膜通量等几个方面对2种膜的分离效果进行了较系统的比较分析。研究表明750 ku空纤维膜羊乳酪蛋白脱除率为98.8%,乳清蛋白脱除率为82.7%;0.1μm卷式有机膜中羊乳酪蛋白脱除率为99.6%,乳清蛋白脱除率为82.7%。过滤后0.1μm卷式有机膜纯水通量衰减系数(39.7%)小于750 ku中空纤维膜(63.7%),前者清洗后纯水通量恢复系数(88.5%)更高,说明卷式有机膜污染慢,易清洗。结果表明,0.1μm卷式有机膜可以有效地分离羊乳中酪蛋白和乳清蛋白,可在工业中大量应用。To quantify the ability of hollow cellulose membranes and rolled organic membranes to separate casein and whey proteins from goat milk,a more systematic comparative analysis of the separation effects of the two membranes was conducted in terms of permeate sensory,SDS-PAGE characterization,Kjeldahl quantification,and membrane flux at the same operating pressure and feed liquid temperature.The study showed that the removal rate of goat milk casein from 750 ku hollow fiber membrane was 98.8%and the removal rate of clear protein was 82.7%;the removal rate of goat milk casein from 0.1μm rolled organic membrane was 99.6%and the removal rate of clear protein was 82.7%.The decay coefficient of pure water flux of 0.1μm rolled organic membrane after filtration(39.7%)was smaller than that of 750 ku hollow fiber membrane(63.7%),and the recovery coefficient of pure water flux after cleaning(88.5%)was higher in the former,indicating that the rolled organic membrane is slow to contaminate and easy to clean.The results suggest that 0.1μm rolled organic membranes can effectively separate casein and whey proteins from goat milk and can be used in large quantities in industry.

关 键 词：羊乳 酪蛋白 乳清蛋白 中空纤维膜 卷式有机膜 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]