响应面法优化栗蘑多糖提取工艺及其抗氧化活性研究  

Optimization of Extraction Process of Polysaccharides from Grifola frondosa by Response Surface Methodology and Study on Their Antioxidant Activity

作　　者：牛牧青 徐婉婷 龙丽芳 吕锐玲 徐碧林 郑永良 NIU Mu-qing;XU Wan-ting;LONG Li-fang;LYU Rui-ling;XU Bi-lin;ZHENG Yong-liang(College of Biology and Agricultural Resources,Huanggang Normal University,Huanggang 438000,China;College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Hubei Key Laboratory of Economic Forest Germplasm mprovement and Resources Comprehensive Utilization,Huanggang 438000,China)

机构地区：[1]黄冈师范学院生物与农业资源学院,湖北黄冈438000 [2]华中农业大学生命科学技术学院,武汉430070 [3]经济林木种质改良及资源综合利用湖北省重点实验室,湖北黄冈438000

出　　处：《中国调味品》2024年第1期183-188,共6页China Condiment

基　　金：国家自然科学基金青年项目(32001207);湖北省中央引导地方科技发展专项资金(2019zyyd044);湖北省重点研发计划项目(2020BBB091)。

摘　　要：为优化栗蘑多糖提取工艺,以栗蘑子实体为研究对象,通过硫酸-苯酚滴定法,结合紫外分光光度法测定栗蘑多糖提取率。在单因素多水平实验的基础上,采用响应面法,利用Design-Expert软件,选取提取时间、提取温度、提取液料比3个因素,设置三水平实验方案优化提取工艺。结果表明,栗蘑多糖最佳提取工艺为提取时间1.9 h、提取温度80℃、提取液料比37∶1(mL/g),此条件下栗蘑多糖提取率为14.45%。抗氧化活性研究结果表明,栗蘑多糖对DPPH自由基和羟基自由基都具有较好的清除能力,在一定范围内,抗氧化活性随着多糖浓度的增加而提高。In order to optimize the extraction process of Grifola frondosa polysaccharides,with Grifola frondosa fruit body as the research object,the extraction rate of polysaccharides from Grifola frondosa is determined by sulfuric acid-phenol titration method combined with ultraviolet spectrophotometry.On the basis of single-factor and multi-level experiment,response surface methodology and Design-Expert software are used to select three factors such as extraction time,extraction temperature and extraction liquid-solid ratio.A three-level experimental scheme is set up to optimize the extraction process.The results show that the optimum extraction conditions are as follows:extraction time is 1.9 h,extraction temperature is 80℃,extraction liquid-solid ratio is 37∶1(mL/g),under such conditions,the extraction rate of polysaccharides is 14.45%.Antioxidant activity research results show that the polysaccharides from Grifola frondosa have good scavenging capacity on DPPH free radical and hydroxyl free radical.Within a certain range,the antioxidant activity increases with the increase of polysaccharides'concentration.

关 键 词：栗蘑 多糖 响应面法 提取工艺 优化 抗氧化活性 

分 类 号：TS201.1[轻工技术与工程—食品科学]