根霉功能曲制曲工艺条件优化研究  

Optimization of Production Process of Functional Rhizopus Starter

作　　者：蒲升惠 秦辉[1,2] 徐琼 张宿义[1,2] 杨艳[1] 蔡小波 黄孟阳[1] 雷雨[1] 刘静 王品翔 付鑫 PU Shenghui;QIN Hui;XU Qiong;ZHANG Suyi;YANG Yan;CAI Xiaobo;HUANG Mengyang;LEI Yu;LIU Jing;WANG Pinxiang;FU Xin(Luzhou Laojiao Co.Ltd.,Luzhou,Sichuan 646000;National Engineering Research Center of Solid-state Brewing,Luzhou,Sichuan 646000,China)

机构地区：[1]泸州老窖股份有限公司,四川泸州646000 [2]国家固态酿造工程技术研究中心,四川泸州646000

出　　处：《酿酒科技》2024年第1期24-29,共6页Liquor-Making Science & Technology

基　　金：四川省科技项目(2021ZYD0102)。

摘　　要：利用中高温大曲中筛选出的高产糖化酶根霉Rhizopus sp.菌株制备功能曲,通过单因素试验及响应面优化设计得出最佳工艺条件为:原料配比小麦粉∶熟糠∶麸皮(36∶10∶5)、接种量2.36%、料水比1∶0.6(g∶mL)、培养温度29.42℃和培养时间54.69 h。在此最佳工艺条件下制备的根霉功能曲糖化力为896 mg/g·h、液化力为1.5 g/g·h、试饭试验还原糖含量为37.5 g/100 g。In this study,Rhizopus sp.strains with high yield of glucoamylase were screened from medium and high-temperature Daqu to produce functional starter.Through single factor tests and response surface optimization design,the optimal process conditions were obtained as follows:the ingredients were wheat flour,rice husk and bran(36∶10∶5),the inoculation amount was 2.36%,the ma-terial-water ratio was 1∶0.6(g∶mL),the cultivation temperature was 29.42℃,and the cultivation time was 54.69 h.Under the above conditions,the saccharification power of the produced functional starter was 896 mg/(g·h),the liquefaction power was 1.5 g/(g·h),and the reducing sugar content in the test was 37.5 g/100 g.

关 键 词：根霉 糖化酶 功能曲 工艺优化 

分 类 号：TS261.4[轻工技术与工程—发酵工程] TS261.1[轻工技术与工程—食品科学与工程]