葛根蛋白酶解物大孔树脂吸附工艺优化及其体外抗氧化活性研究  

Optimization of macroporous resin adsorption process of enzymatic hydrolysis of Pueraria protein and its antioxidant activity in vitro by response surface method

作　　者：岳奕含 严铭铭[1] 邵帅[1] 丁奕元 江婷 YUE Yihan;YAN Mingming;SHAO Shuai;DING Yiyuan;JIANG Ting(Changchun University of Chinese Medicine,Changchun,Jilin 130117,China)

机构地区：[1]长春中医药大学,吉林长春130117

出　　处：《食品与机械》2024年第1期167-174,共8页Food and Machinery

基　　金：吉林省科技发展计划项目(编号:20210401111YY);吉林省发改委产业技术研究与开发项目(编号:2023C027-4);吉林省教育厅人文社科研究项目(编号:JJKH20211006SK)。

摘　　要：目的:优化大孔树脂吸附葛根蛋白酶解物工艺及其抗氧化活性研究。方法:采用Box-Behnken响应面法确定大孔树脂吸附葛根蛋白酶解物最佳工艺。以维生素C为对照,测定最佳工艺下纯化前后葛根蛋白酶解物的抗氧化活性。结果:大孔树脂的最佳吸附—解吸工艺为上样液质量浓度10.0 mg/mL,洗脱液流速2.6 mL/min,洗脱液乙醇体积分数74%,纯化后葛根蛋白酶解物含量提升至37.19%。大孔树脂纯化后的葛根蛋白酶解物对DPPH自由基、ABTS+自由基、羟自由基的清除率均强于吸附前的。结论:大孔树脂纯化后的葛根蛋白酶解物抗氧化效果良好,可以作为一种潜在的蛋白多肽应用于食品中。Objective:This study aims to optimize the adsorption of enzymatic hydrolysis of Pueraria protein by macroporous resin and maximize the antioxidant properties in vitro.Methods:The Box-Behnken response surface method was used to determine the optimization of adsorption of enzymatic hydrolysis of Pueraria protein.Using V C as a control,the antioxidant activity of Kase was determined before and after purification under the optimal purification process.Results:The optimal adsorption-desorption process of macroporous resin is:the mass concentration of the sample solution was 10.0 mg/mL,the flow rate of the eluent was 2.6 mL/min,and the volume fraction of ethanol in the eluent was 74%.After adsorption,the content of enzymatic hydrolysis of Pueraria protein increased to 37.19%.The scavenging rate of DPPH,ABTS+,and hydroxyl radicals of Pueraria Mirifica protease after adsorption was stronger than before adsorption.Conclusion:This study shows that enzymatic hydrolysis of Pueraria protein after adsorption of macroporous resin has good antioxidant effect,which can be used as a potential protein polypeptide in food,providing data reference for further research of enzymatic hydrolysis of Pueraria protein.

关 键 词：葛根蛋白 酶解物 大孔树脂 抗氧化活性 吸附 

分 类 号：TS201.2[轻工技术与工程—食品科学]