5种香蕉果肉多酚的抗氧化活性和抑制α-葡萄糖苷酶活性  

Antioxidant activity andα-glucosidase inhibitory activity of five banana pulp polyphenols

作　　者：冉静凤 陈小爱 易翠平[1] 张彦军[2,3,4,5] RAN Jingfeng;CHEN Xiaoai;YI Cuiping;ZHANG Yanjun(School of Food Science and Bioengineering,Changsha University of Science and Technology,Changsha,Hunan 410114,China;Spice and Beverage Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wanning,Hainan 571533,China;National Center of Important Tropical Crops Engineering and Technology Research,Wanning,Hainan 571533,China;Key Laboratory of Processing Suitability and Quality Control of the Special Tropical Crops of Hainan Province,Wanning,Hainan 571533,China;Hainan Provincial Engineering Research Center of Tropical Spice and Beverage Crops,Wanning,Hainan 571533,China)

机构地区：[1]长沙理工大学食品与生物工程学院,湖南长沙410114 [2]中国热带农业科学院香料饮料研究所,海南万宁571533 [3]国家重要热带作物工程技术研究中心,海南万宁571533 [4]海南省特色热带作物适宜性加工与品质控制重点实验室,海南万宁571533 [5]海南省热带香料饮料作物工程技术研究中心,海南万宁571533

出　　处：《食品与机械》2023年第12期153-161,共9页Food and Machinery

基　　金：海南省重点研发计划项目(编号:ZDYF2022SHFZ122);中国热带农业科学院基本科研业务费专项资金(编号:1630142022007)。

摘　　要：目的:比较不同品种香蕉果肉多酚的活性差异。方法:超声萃取5种香蕉果肉多酚,利用超高效液相色谱分析酚类组成,分析其抗氧化能力和对α-葡萄糖苷酶活性的抑制能力。结果:5种香蕉果肉间的多酚含量、黄酮含量和单宁含量差异显著(P<0.05);在5种香蕉果肉中检测出8种主要单体酚,包括4种酚酸及其衍生物和4种黄酮类化合物;5种香蕉果肉多酚具有一定的DPPH自由基清除能力、ABTS自由基清除能力和铁离子还原能力,均能抑制α-葡萄糖苷酶活性,主要活性成分芦丁、儿茶素与抑制能力呈显著正相关(P<0.05)。南角42号香蕉果肉多酚的抗氧化能力和抑制α-葡萄糖苷酶活性的能力最强,通过混合型抑制的方式抑制酶活,其与α-葡萄糖苷酶的相互作用为放热反应。结论:香蕉果肉多酚具有很好的抗氧化能力和对α-葡萄糖苷酶活性的抑制能力,有望成为良好的α-葡萄糖苷酶选择性抑制剂。Objective:The purpose of this study was to compare the activity difference of polyphenols in banana pulp of different varieties and to provide scientific basis for the exploitation and utilization of banana pulp polyphenols.Methods:Five banana pulp polyphenols were extracted by ultrasonic extraction,and the phenolic components were analyzed by ultra-high performance liquid chromatography(UHPLC).The antioxidant capacity and inhibition ability ofα-glucosidase were determined.Results:The total polyphenols,flavonoids and tannins content were significant differences among five varieties(P<0.05).Eight phenolic compounds were identified in five banana pulps,including four phenolic acids and their derivatives,and four flavonoid compounds.The five kinds of banana pulp polyphenols had certain antioxidant activities of scavenging DPPH,ABTS free radical,and reducing iron ion.All the five kinds of banana pulp polyphenols could inhibitα-glucosidase.The major bioactive components in banana pulp,catechin acid and rutin,were signi cantly positively correlated with the ability ofα-glucosidase inhibition(P<0.05).The antioxidative ability and inhibitory activity ofα-glucosidase were the strongest in the pulp polyphenols of Nanjiao No.42 banana.The enzyme activity was inhibited by mixed inhibition,and the interaction withα-glucosidase was exothermic.Conclusion:Banana pulp polyphenols have good antioxidant ability and inhibition ability toα-glucosidase activity,and are expected to be a good selective inhibitor ofα-glucosidase.

关 键 词：香蕉果肉 品种 多酚 体外抗氧化能力 α-葡萄糖苷酶抑制活性 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]