超声波辅助复合酶法提取Iota卡拉胶工艺优化  

Optimization of extraction technology of Iota carrageenan by ultrasonic wave combined with enzyme

作　　者：谢立梅 陈静 郑慧新[1,2] 郭少莉 张宁宁 XIE Limei;CHEN Jing;ZHENG Huixin;GUO Shaoli;ZHANG Ningning(College of Food Science,Fujian Agricultural and Forestry University,Fuzhou,Fujian 350002,China;Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition,Ministry of Education,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]闽台特色海洋食品加工及营养健康教育部工程研究中心,福建福州350002

出　　处：《食品与机械》2023年第12期171-178,共8页Food and Machinery

基　　金：福建省海洋经济发展专项资金项目(编号:FJHJF-L-2021-1)。

摘　　要：目的:优化Iota卡拉胶提取工艺,以期提高Iota卡拉胶产率。方法:采用超声波辅助复合酶技术从刺麒麟菜中提取Iota卡拉胶。通过单因素和正交试验,确定最佳复合酶(纤维素酶、半纤维素酶、木瓜蛋白酶)配比和复合酶酶解条件,并优化超声处理条件和煮胶工艺条件。结果:最佳复合酶配比为纤维素酶0.50%、半纤维素酶1.25%、木瓜蛋白酶0.20%;最佳酶解条件为酶解温度60℃,酶解pH 5.5、酶解时间2.5 h、酶解料液比1∶30 (g/mL);最佳超声处理工艺为超声功率350 W、超声时间25 min、超声温度40℃;最佳煮胶工艺为煮胶温度90℃、六偏磷酸钠(SHMP)添加量0.04%、煮胶时间3 h。结论:在最优工艺条件下,提取的Iota卡拉胶产率高,可达到47.17%。Objective:This study focused on optimizing the Iota carrageenan extraction process and improving Iota carrageenan yield.Methods:Iota carrageenan was extracted from Eucheuma spinulosa by ultrasonic-assisted complex enzyme technology.Through single factor and orthogonal experiments,the optimum ratio of complex enzymes(cellulase,hemicellulose and papain)and enzymatic hydrolysis conditions of complex enzymes were first determined.Then,the optimum ultrasonic treatment conditions were determined.Finally,the boiling process conditions were optimized.Results:The optimal compound enzyme ratios were cellulase of 0.50%,hemicellulase of 1.25%and papain of 0.20%.The optimal enzymolysis conditions were as follows:enzymolysis at 60℃and pH 5.5 for 2.5 h,with enzymolysis material-liquid ratio at 1∶30(g/mL).The best ultrasonic treatment process was ultrasonic power of 350 W,treated at 40℃for 25 min.The best boiling process was using 0.04%sodium hexametaphosphate(SHMP),boiling at 90℃for 3 h.Conclusion:Under the control of the optimal process conditions,Iota carrageenan can reach a high extraction yield as 47.17%.

关 键 词：刺麒麟菜 Iota卡拉胶 超声波 纤维素酶 半纤维素酶 木瓜蛋白酶 

分 类 号：TS201.7[轻工技术与工程—食品科学]